The role of DNA binding with the Cry8Ea1 toxin of Bacillus thuringiensis

文献类型： 外文期刊

第一作者： Song, Fuping

作者： Song, Fuping;Zhang, Jie;Guo, Shuyuan;Li, Jie

作者机构：

关键词： δ-endotoxin;Aggregation;Bacillus thuringiensis;Protoxin-DNA;Toxin-DNA

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The Cry8Ea1 protoxin is a DNA-protein complex. Both forms of the Cry8Ea1 toxin (with or without DNA binding) were obtained separately, and their stability and ability to insert into a phospholipid monolayer in vitro were compared. The presence of DNA can prevent the toxin from aggregation. Data regarding the penetration of the Cry8Ea1 toxin and Cry8Ea1 toxin-DNA complex into the air/water interface without a phospholipid monolayer show that the Cry8Ea1 toxin-DNA complex is more likely to move towards the air/water interface and is more hydrophobic. Experiments examining the protein interaction with the phospholipid monolayer show that the ability of the Cry8Ea1 toxin-DNA complex to insert into the lipid bilayer is much greater than that of the Cry8Ea1 toxin without DNA.

分类号： Q93

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