Synthesis and characterization of folate conjugated chitosan and cellular uptake of its nanoparticles in HT-29 cells

文献类型： 外文期刊

第一作者： Li, Puwang

作者： Li, Puwang;Wang, Yichao;Kong, Ling Xue;Li, Puwang;Peng, Zheng;Zeng, Fanbo;Chen, Lijue

作者机构：

关键词： Cell uptake;Chemical modification;Chitosan;Folate;HT-29 cells

期刊名称：CARBOHYDRATE RESEARCH （ 影响因子：2.104； 五年影响因子：2.192 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Folate-chitosan (FA-CS) conjugates synthesized by coupling FA with CS render new and improved functions because the original properties of CS are maintained and the targeting ligand of FA is incorporated. In this work, FA-CS conjugates were synthesized based on chemical linking of carboxylic group of FA with amino group of CS as confirmed by Fourier transform spectroscopy (FTIR) and nuclear magnetic resonance (~1H NMR). FA-CS conjugates displayed less crystal nature when compared to CS. The FA-CS nanoparticles (NPs) were prepared by crosslinking FA-CS conjugates with sodium tripolyphosphate (STPP). Positively charged FA-CS nanoparticles were spherical in shape with a particle size of about 100 nm. Cellular uptake of CS or FA-CS nanoparticles was assayed by fluorescent microscopy using calcein as fluorescent marker in colon cancer cells (HT-29). The FA-CS nanoparticles exhibited improved uptake of HT-29 and could become a potential targeted drug delivery system for colorectal cancer.

分类号： O62

  • 相关文献

[1]Development of Ligand Incorporated Chitosan Nanoparticles for the Selective Delivery of 5-Fluorouracil to Colon. Li, Puwang,She, F. H.,Kong, L. X.,Li, Puwang,Li, Puwang,Peng, Zheng. 2011

[2]Preparation of nanoparticles by crosslinking folate conjugated chitosan with vanillin and its characterization. Zhou, Min,Li, Puwang,Wang, Guang,Yang, Ziming,Peng, Zheng,Kong, Lingxue. 2012

[3]Synthesis and characterization of chitosan quaternary ammonium salt and its application as drug carrier for ribavirin. Li, Si-Dong,Quan, Wei-Yan,Yang, Lei,Dong, Jing-Jing,Li, Pu-Wang,Yang, Zi-Ming,Peng, Zheng,Yang, Xi-Hong.

[4]Arabidopsis plastidial folylpolyglutamate synthetase is required for nitrogen metabolism under nitrate-limited condition in darkness. Meng, Hongyan,Xu, Bosi,Zhang, Chunyi,Jiang, Ling,Zhang, Chunyi,Jiang, Ling.

[5]Folate supplementation modifies CCAAT/enhancer-binding protein alpha methylation to mediate differentiation of preadipocytes in chickens. Wen, Jie.

[6]Protocatechualdehyde Induces S-Phase Arrest and Apoptosis by Stimulating the p27(KIP1)-Cyclin A/D1-CDK2 and Mitochondrial Apoptotic Pathways in HT-29 Cells. Zhong, Shi,Li, You-Gui,Ji, Dong-Feng,Lin, Tian-Bao,Lv, Zhi-Qiang.

[7]Immunomodulatory effects of Clostridium butyricum on human enterocyte-like HT-29 cells. Gao Quanxin,Qi Lili,Wu Tianxing,Xia Tingting,Wang Jinbo,Gao Quanxin.

[8]Induction of apoptosis in HT-29 cells by quercetin through mitochondria-mediated apoptotic pathway. Li, Zhaohui,Gao, Quanxin.

[9]Preparation and Characterization of a Novel Antibacterial Fiber Modified by Quaternary Phosphonium Salt on the Surface of Polyacrylonitrile Fiber. Chang, Li,Liu, Xuanming,Zhang, Xiaozhuan,Zhao, Liang,Shi, Xibao. 2014

[10]Sorption of mercury (II) and atrazine by biochar, modified biochars and biochar based activated carbon in aqueous solution. Tan, Guangcai,Xu, Yaru,Xu, Nan,Sun, Weiling,Wang, Hongyuan.

[11]A Fungal Alpha-Galactosidase from Pseudobalsamia microspora Capable of Degrading Raffinose Family Oligosaccharides. Yang, Dongxue,Du, Fang,Wang, Hexiang,Yang, Dongxue,Du, Fang,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Tzi Bun Ng.

[12]Comparison of effect of chitin, chitosan, chitosan oligosaccharide and N-acetyl-D-glucosamine on growth performance, antioxidant defenses and oxidative stress status of Penaeus monodon. Niu, Jin,Lin, Hei-Zhao,Jiang, Shi-Gui,Chen, Xu,Wu, Kai-Chang,Liu, Yong-Jian,Tian, Li-Xia,Wang, Sheng. 2013

[13]Development and characterization of nano-bilayer films composed of polyvinyl alcohol, chitosan and alginate. Zhuang, Chenjun,Jiang, Yongli,Zhong, Yu,Zhao, Yanyun,Deng, Yun,Yue, Jin,Wang, Danfeng,Jiao, Shunshan,Zhao, Yanyun,Gao, Haiyan,Chen, Hangjun,Mu, Honglei. 2018

[14]EFFECTS OF CHITOSAN AND MICROFLUIDIZATION PRETREATMENT ON PHYSICOCHEMICAL AND MECHANICAL PROPERTIES OF VICILIN-RICH PROTEIN ISOLATE (PHASEOLUS AUREUS) FILMS. Huang, L. H.,Zhang, Y. H.. 2015

[15]Advances in characterisation and biological activities of chitosan and chitosan oligosaccharides. Zou, Pan,Yang, Xin,Wang, Jing,Zhang, Yanxin,Liu, Guangyang,Zou, Pan,Yang, Xin,Wang, Jing,Li, Yongfei,Yu, Hailong,Zhang, Yanxin,Liu, Guangyang,Wang, Jing,Li, Yongfei,Yu, Hailong. 2016

[16]Effect of electrostatically charged and neutral polysaccharides on the rheological characteristics of peanut protein isolate after high-pressure homogenization. Jiao, Bo,Shi, Aimin,Liu, Hongzhi,Sheng, Xiaojing,Liu, Li,Hu, Hui,Wang, Qiang,Adhikari, Benu. 2018

[17]Chitosan extends the shelf-life of filleted tilapia (Oreochromis niloticus) during refrigerated storage. Cao Rong,Liu Qi,Yin Bangzhong,Wu Biao. 2012

[18]Preparation and characterization of alpha-galactosidase-loaded chitosan nanoparticles for use in foods. Liu, Yong,Li, Yanli,Xu, Shaochun,Tang, Jiangwu,Xu, Yaoxing,Sun, Yan,Ding, Juntao. 2011

[19]Effect of chitosan solution on the inhibition of Acidovorax citrulli causing bacterial fruit blotch of watermelon. Li, Bin,Shi, Yu,Shan, Changlin,Zhou, Qing,Ibrahim, Muhammad,Li, Hongye,Xie, Guanlin,Wang, Yanli,Sun, Guochang,Wu, Guoxing. 2013

[20]Characteration of Protein Loaded Chitosan Nanoparticles at Different pH Values. Liu, Yong,Li, Yanli,Xu, Shaochun,Xu, Yaoxing,Sun, Yan. 2011

作者其他论文 更多>>

微信小程序