Development of a plant regeneration system from seed-derived calluses of centipedegrass [Eremochloa ophiuroides (Munro.) Hack]

文献类型： 外文期刊

第一作者： Yuan, Xuejun

作者： Yuan, Xuejun;Wang, Zhiyong;Liu, Jianxiu;Yuan, Xuejun;She, Jianming

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关键词： Eremochloa ophiuroides;MH - lawns and turf;MH - turf grasses;MH - micropropagation;MH - callus;MH - seeds;MH - explants;MH - culture media;MH - mannitol;MH - coconut milk;MH - benzyladenine;MH - naphthaleneacetic acid;MH - rooting;MH - shoots;MH - in vitro regeneration

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

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收录情况： SCI

摘要： The objective of this study is to establish plant regeneration system with the seed of the new Chinese selection E-126of centipedegrass [Eremochloa ophiuroides (Munro.) Hack] as explant. In present study, the following results were obtained: (1) The medium formulation most suitable for calluses induction was identified to be MS with 1.0mglp# 2,4-D+30glp# mannitol+50mllp# coconut milk and the ratio of calluses induction was 96.0%, including 5.2% of yellow granule calluses induction. The above medium formulation was adopted for subculture. (2) The rate of shoot regeneration from yellow granular calluses was 98.0% by MS optimum medium formulation with 2.0mglp# KT+50mllp# coconut milk. The differentiated rate retained as high as 88.0% even after 5 times of subculture and 18.6% after 15 times of subculture. The optimum medium formulation for shoot growth was identified to be MS medium plus 2.0mglp# BAP, 0.8mglp# NAA and 50mllp# coconut milk. (3) The optimum medium for shoot rooting was identified to be MS medium with 0.6mglp# NAA+50mllp# coconut milk, and the rooting rate to be 98.0%. The survival rate of transplanted plantlets from tubes to basin with soil was 92.0%. In conclusion, the plant regeneration system was successfully developed in this study, which may provide basic reference for screening of somaclonal variants and genetic transformation of centipedegrass.

分类号： S6

  • 相关文献

[1]High-Efficiency Plant Regeneration from Immature Inflorescence Derived Callus Cultures of Two Phenotypically Distinct Accessions of Centipedegrass (Eremochloa ophiuroides). Ma, Jingjing,Wang, Yi,Zong, Junqin,Zhang, Bing,Chen, Jingbo,Li, Dandan,Li, Ling,Guo, Hailin,Liu, Jianxiu,Li, Jianjian,She, Jianming. 2018

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