Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk

文献类型： 外文期刊

第一作者： Li, Yangrui

作者： Li, Yangrui;Yang, Litao

作者机构：

关键词： sucrose: 57-50-1;methyl jasmonate: 1211-29-6;activated charcoal;6-benzyladenine: 1214-39-7;paclobutrazol: 76738-62-0;Murashige and Skoog medium: MS medium;naphthalene acetic acid: NAA;26445-01-2;shoot proliferation;shoot dry weight;shoot fresh weight;shoot tuberization

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Dioscorea fordii Prain et Burk., a member of the family Dioscoreaceae, is an important tuberous food in China owing to its edible and medicinal functions. However, the lack of healthy planting material has restricted its production. The present study was an attempt to develop a protocol for in vitro propagation of D. fordii. In an initial two by two (2(2)) factorial experiment, the effects of culture system and activated charcoal have been observed for axillary shoot proliferation and tuberization of in vitro plantlets. Shoot length, frequency of proliferation, fresh weight and dry weight of shoots, frequency of tuberization and mean number of tubers per plantlet (NTPs) were significantly (P a parts per thousand currency sign 0.05) greater in liquid medium compared to semi-solid media. Secondly, an orthogonal experimental design [L-9 (3(4))] was used to investigate the effects of the ratio of naphthalene acetic acid (NAA) to 6-benzyladenine (BA) (NAA/BA), paclobutrazol, methyl jasmonate and sucrose concentration on tuberization. Sucrose concentration had the record effect on frequency of tuberization, NTPs and frequency of proliferation. The preferred medium for axillary shoot proliferation and tuberization of D. fordii found to be MS basal medium (Murashige and Skoog in Physiol Plant 15:473-479, 1962) supplemented with 1.0 mg l(-1) BA, 0.1 mg l(-1) NAA, 30 g l(-1) sucrose and 1.5 g l(-1) AC in liquid culture.

分类号： Q942

  • 相关文献

[1]Studies on reproductive characteristics of an interspecific chimera between Brassica juncea and Brassica oleracea. Wang, Yan,Zhu, Xue-Yun,Chen, Li-Ping,Cheng, Qi,Wang, Yan,Chen, Li-Ping.

[2]Agrobacterium-mediated transformation of Eupatorium adenophorum. Guo, Huiming,Zhang, Yongqiang,Cheng, Hongmei,Wan, Fanghao.

[3]Impact of genotype, plant growth regulators and activated charcoal on embryogenesis induction in microspore culture of pepper (Capsicum annuum L.). Cheng, Yan,Ma, Rong-li,Jiao, Yan-sheng,Qiao, Ning. 2013

[4]Plant Regeneration from Shoot Tips in Triploid Loquat. Wang, Y. Q.,Liu, J.,Deng, Q. X.,Tao, L..

[5]Gene expression pattern in response to wounding, methyl jasmonate and ethylene in the bark of Hevea brasiliensis. Duan, Cuifang,Rio, Maryannick,Leclercq, Julie,Oliver, Gerald,Montoro, Pascal,Duan, Cuifang,Bonnot, Francois.

[6]Molecular Dissection of the Pathogen-Inducible 3-Deoxyanthocyanidin Biosynthesis Pathway in Sorghum. Liu, Hongjia,Du, Yegang,Chu, Hung,Shih, Chun Hat,Wong, Yu Wai,Wang, Mingfu,Lo, Clive,Liu, Hongjia,Tao, Yuezhi,Chu, Ivan K..

[7]Mapping QTLs for nitrogen-deficiency tolerance at seedling stage in rice (Oryza sativa L.). Cao, L. Y.,Wu, W. M.,Shen, X. H.,Zhan, X. D.,Zhai, R. R.,Wang, R. C.,Chen, D. B.,Cheng, S. H.,Feng, Y..

[8]Comparisons of cadmium tolerance and accumulation at seedling stage in wheat varieties grown in different decades in China. Jiang, Dong,Ci, Dunwei,Jiang, Dong,Dai, Tingbo,Cao, Weixing,Liu, Fulai,Ci, Dunwei.

[9]Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana. Qinghua Wang,Xue Zhang,Fuguang Li,Yuxia Hou,Xingliang Liu,Xueyan Zhang.

[10]Characterization of a Glucose-, Xylose-, Sucrose-, and d-Galactose-Stimulated beta-Glucosidase from the Alkalophilic Bacterium Bacillus halodurans C-125. Xu, Hu,Chen, Jian-Min,Xu, Hu,Xiong, Ai-Sheng,Zhao, Wei,Tian, Yong-Sheng,Peng, Ri-He,Yao, Quan-Hong.

[11]Effect of 1-MCP treatment on nutritive and functional properties of loquat fruit during cold storage. Cao, S.,Zheng, Y.,Yang, Z.,Cao, S..

[12]Prevalence of Cryptosporidium spp. in pigs in Shanghai, China. Chen, Zhaoguo,Mi, Rongsheng,Yu, Huizhu,Shi, Yaojun,Huang, Yan,Chen, Yongjun,Zhou, Peng,Cai, Youmin,Lin, Jiaojiao.

[13]Characterization of biofilm formation by Riemerella anatipestifer. Hu, Qinghai,Han, Xiangan,Zhou, Xiaojin,Ding, Chan,Yu, Shengqing,Ding, Siyu.

[14]The myb transcription factor MdMYB6 suppresses anthocyanin biosynthesis in transgenic Arabidopsis. Gao, Jian-Jie,Shen, Xue-Fang,Peng, Ri-He,Xiong, Ai-Sheng,Zhu, Bo,Yao, Quan-Hong,Gao, Jian-Jie,Shen, Xue-Fang,Zhang, Zhen,Xu, Jing,Zheng, Jian-Li,Yao, Quan-Hong,Yao, Quan-Hong.

[15]Isolation and Identification of a Pathogen of Silkworm Bombyx mori. Tao, Heng-Ping,Shen, Zhong-Yuan,Zhu, Feng,Xu, Xiao-Fang,Tang, Xu-Dong,Shen, Zhong-Yuan,Xu, Li.

[16]Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane. Upadhyay, Santosh K.,Verma, Praveen C.,Verma, Ashok K.,Singh, S. B.,Solomon, S.,Srivastava, Manoj K..

[17]Single amino acid residue changes in subsite-1 of levansucrase from Zymomonas mobilis 10232 strongly influence the enzyme activities and products. Li, Shuying,Yan, Yongliang,Zhou, Zhengfu,Yu, Haiying,Zhan, Yuhua,Zhang, Wei,Chen, Ming,Lu, Wei,Ping, Shuzhen,Lin, Min.

作者其他论文 更多>>

微信小程序