Cloning of equine herpesvirus type 1 438/77 strain genome as a bacterial artificial chromosome

文献类型: 外文期刊

第一作者: Sun, Xuegiang

作者: Sun, Xuegiang;Yao, Huochun;Lu, Chengping;Zhang, Cun

作者机构:

关键词: EHV-1;herpesvirus 438/77;recombinant virus;bacterial artificial chromosome;BAC;EHV-1;Herpesvirus 438/77;Rekombinantes Virus;bakterielles artifizielles Chromosome;BAC

期刊名称:BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT ( 影响因子:0.328; 五年影响因子:0.488 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Equine herpesvirus type 1 (EHV-1) is a major cause of respiratory and reproductive diseases in horses worldwide. The genome of EHV-1 strain 438/77 (isolated from an aborted equine fetus) was cloned as a bacterial artificial chromosome (BAC) in £ coliwithout any gene deletions. The mini-F plasmid sequence was inserted in the middle of ORF19 and 20 via homologous recombination following co-transfection of viral DNA and plasmid pE19_20/HA into RK13 cells. Circular viral DNA was extracted from RK13 cells infected with purified recombinant virus expressing green fluorescent protein (GFP) and electrophorated into E coli DH10B cells. The clone harboring the BAC was screened and analyzed by PCR and RFLR Reconstitution of the recombinant virus was achievedsuccessfully by transfec-tion of the BAC DNA into RK13 cells. The mini-F sequence in the reconstituted virus was subsequently removed by homologous recombination between virus DNA and plasmid pE1920XM, inducing a point mutation in the Xbal site in ORF19. Comparison of RFLP profiles of the rescued, recovered and the wild-type viral genome demonstrated that no unexpected changes occurred during mutagenesis. In vitro replication assays showed that BAC-reconstituted virus mutant growth kinetics and plaqueformation morphology/size were indistinguishable to those measured for wild-type virus.

分类号: S85

  • 相关文献

[1]Development of a single-tube duplex EvaGreen real-time PCR for the detection and identification of EHV-1 and EHV-4. Hu, Zhe,Zhu, Chao,Chang, Hao,Guo, Wei,Liu, Diqiu,Xiang, Wenhua,Wang, Xiaojun.

[2]A Gossypium BAC clone contains key repeat components distinguishing sub-genome of allotetraploidy cottons. Yuling Liu,Renhai Peng,Fang Liu,Xingxing Wang,Xinglei Cui,Zhongli Zhou,Chunying Wang,Xiaoyan Cai,Yuhong Wang,Zhongxu Lin,Kunbo Wang. 2016

[3]Screening and chromosome localization of two cotton BAC clones. Xinglei Cui,Fang Liu,Yuling Liu,Zhongli Zhou,Chunying Wang,Yanyan Zhao,Fei Meng,Xingxing Wang,Xiaoyan Cai,Yuhong Wang,Renhai Peng,Kunbo Wang. 2016

[4]Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts. Shao Changwei,Shao Changwei,Liu Shanshan,Liu Changlin,Chen Songlin,Liu Geng. 2013

[5]Deletion of the BAC sequences from recombinant meq-null Marek's disease (MD) virus increases immunosuppression while maintaining protective efficacy against MD. Su, S.,Cui, N.,Sun, P.,Cui, Z.,Li, J.,Li, H.,Li, Y..

[6]Cytogenetic maps of homoeologous chromosomes A(h)01 and D(h)01 and their integration with the genome assembly in Gossypium hirsutum. Yuling Liu,Liu, Fang,Wang, Kunbo,Zhen Liu,Renhai Peng,Yuhong Wang,Zhongli Zhou,Xiaoyan Cai,Xingxing Wang,Zhenmei Zhang,Kunbo Wang,Fang Liu. 2017

[7]Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization. Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,You, Weiwei,Ke, Caihuan,Zhang, Xiaojun,Jiang, Likun,Chen, Baohua,Mahboob, Shahid,Al-Ghanim, Khalid A.,Mahboob, Shahid.

[8]Virus-encoded miR-155 ortholog is an important potential regulator but not essential for the development of lymphomas induced by very virulent Marek's disease virus. Yu, Zu-Hua,Teng, Man,Yu, Le-Le,Hu, Bo,Zhang, Gai-Ping,Luo, Jun,Yu, Zu-Hua,Hu, Bo,Liu, Ju-Xiong,Zhang, Gai-Ping,Sun, Ai-Jun,Cui, Zhi-Zhong,Qu, Liang-Hu,Yu, Zu-Hua,Ding, Ke,Cheng, Xiang-Chao,Luo, Jun.

[9]Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library. Xia, Zhengjun,Wu, Hongyan,Xia, Zhengjun,Watanabe, Satoshi,Harada, Kyuya.

[10]An inactivated gE-deleted pseudorabies vaccine provides complete clinical protection and reduces virus shedding against challenge by a Chinese pseudorabies variant. Wang, Jichun,Guo, Rongli,Qiao, Yongfeng,Xu, Mengwei,Wang, Zhisheng,Liu, Yamei,Gu, Yiqi,Liu, Chang,Hou, Jibo,Gu, Yiqi,Liu, Chang,Wang, Jichun,Guo, Rongli,Qiao, Yongfeng,Xu, Mengwei,Wang, Zhisheng,Liu, Yamei,Hou, Jibo. 2016

[11]A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge. Tian, ZJ,Zhou, GH,Zheng, BL,Qiu, HJ,Ni, JQ,Yang, HL,Yin, XN,Hu, SP,Tong, GZ. 2006

[12]Construction of a full-length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain. Chen, Liu,Yu, Bin,Hua, Jionggang,Ye, Weicheng,Ni, Zheng,Yun, Tao,Deng, Xiaohui,Zhang, Cun. 2013

[13]Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat. Kong, XY,Gu, YQ,You, FM,Dubcovsky, J,Anderson, OD.

[14]A recombinant porcine circovirus type 2 expressing the VP1 epitope of the type O foot-and-mouth disease virus is infectious and induce both PCV2 and VP1 epitope antibodies. Huang, Liping,Zhang, Feiyan,Tang, Qinghai,Wei, Yanwu,Wu, Hongli,Guo, Longjun,Fu, Yujie,Liu, Changming.

[15]Development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of S1 gene of H120 strain. Wei, Yan-Quan,Guo, Hui-Chen,Dong, Hu,Wang, Hai-Ming,Xu, Jin,Sun, De-Hui,Cai, Xue-Peng,Liu, Ding-Xiang,Sun, Shi-Qi,Liu, Ding-Xiang,Fang, Shou-Guo.

[16]Recombinant Gallid herpesvirus 2 with interrupted meq genes confers safe and efficacious protection against virulent field strains. Zhang, Yanping,Liu, Changjun,Yan, Fuhai,Liu, Ailing,Cheng, Yun,Li, Zhijie,Sun, Guorong,Lv, Hongchao,Wang, Xiaomei.

[17]Recombinant canine adenovirus type-2 expressing TgROP16 provides partial protection against acute Toxoplasma gondii infection in mice. Li, Xiu-zhen,Lv, Lin,Abdullahi, Auwalu Yusuf,Tu, Liqing,Xia, Lijun,Feng, Weili,Li, Shoujun,Yuan, Zi-Guo,Li, Xiu-zhen,Lv, Lin,Abdullahi, Auwalu Yusuf,Tu, Liqing,Xia, Lijun,Feng, Weili,Lu, Chunxia,Li, Shoujun,Yuan, Zi-Guo,Zhang, Xu,Anchang, Kenneth Yongabi,Wang, Xiaohu,Zhang, Xiu-Xiang.

[18]Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer. Chai, Zheng,Fu, Fang,Zhang, Xueyun,Li, Xi,Zhang, Peiyi,Liu, Ying,Hu, Lihua. 2014

[19]A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity. Sun AiJun,Cui ZhiZhong,Lawrence, Petherbridge,Zhao YuGuang,Nair, Venugopal K.,Li YanPeng. 2009

[20]Identification of sesame (Sesamum indicum L.) chromosomes using the BAC-FISH system. Zhao, R.,Song, W.,Chen, C.,Zhao, R.,Miao, H.,Zhang, H.. 2018

作者其他论文 更多>>