Injection of frozen-thawed porcine first polar bodies into enucleated oocytes results in fertilization and embryonic development

文献类型： 外文期刊

第一作者： Wang, G. J.

作者： Wang, G. J.;Yu, J. N.;Xu, X. B.;Fan, B. Q.;Tan, X. D.;Zhou, X. L.

作者机构：

关键词： embryonic development;developmental competence;fertilization process;genetic difference;developmental research;frozen-thawed polar body;recombinant zygote

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The objective was to determine whether enucleated oocytes injected with frozen porcine first polar bodies (pPB1s) could be fertilized and developed into viable embryos in vitro. Metaphase II (MII) oocytes with pPB1s were frozen (vitrified) and stored for 2 mo. The pPB1s were isolated from thawed MII oocytes and injected into enucleated recipient oocytes by micromanipulation. All recipients injected with thawed pPB1s were fertilized by intracytoplasmic sperm injection (ICSI), and the resulting recombinant zygotes were incubated to assess their developmental competence in vitro. Furthermore, double-antibody immunohistochemistry was used to verify that the nucleus of the pPB1 participated in fertilization and supported embryonic development. Porcine embryos (2- to 8-cell stage) were obtained from the recombinants. The average in vitro cleavage rate of 2-, 4-, and 8-cell stage recombinant embryos was 25.3, 17.7, and 9.3% (P < 0.05), respectively. Chromosomes in the labeled pPB1 participated in the formation of the two blastomere nuclei of 2-cell stage embryos derived from recombinant oocytes. In conclusion, nuclear materials of frozen-thawed pPB1 supported oocyte fertilization and subsequent embryonic development, thereby providing a new way to use frozen PB1s for preservation and reproduction of mammals

分类号： Q95

  • 相关文献

[1]Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa. Xiao, Ling,Yu, Deyue,Xiao, Ling,Lu, Changming,Bo, Huijie,Wu, Yuhua,Wu, Gang,Cao, Yinglong,Zhang, Bing.

[2]The genetic diversity and origin of Chinese game chicken. Zhu, Wenqi,Li, Huifang,Song, Weitao,Xu, Wenjuan,Shu, Jingting,Han, Wei,Chen, Kuanwei.

[3]A Comparison of in vitro Development of Buffalo (Bubalus bubalis) Embryos Produced either by Somatic Cell Nuclear Transfer or in vitro Fertilization using Oocytes Obtained by Different Methods. Yang, Chunyan,Pang, Chunying,Zheng, Haiying,Huang, Fenxiang,Shang, Jianghua,Wang, Jian,Yang, Bingzhuang,Liang, Xianwei. 2013

[4]Study on the inter-subspecies nuclear transfer of river buffalo somatic cell nuclei into swamp buffalo oocyte cytoplasm. Yang, C. Y.,Li, R. C.,Pang, C. Y.,Yang, B. Z.,Qin, G. S.,Chen, M. T.,Zhang, X. F.,Huang, F. X.,Zheng, H. Y.,Huang, Y. J.,Liang, X. W..

[5]Effects of SOF and CR1 media on developmental competence and cell apoptosis of ovine in vitro fertilization embryos. Wan, Peng-cheng,Hao, Ze-dong,Wu, Yi,Yang, Ling,Cui, Mao-sheng,Zeng, Shen-ming,Wan, Peng-cheng,Zhou, Ping,Liu, Shou-ren.

[6]BmARM-Like Protein from Silkworm, Bombyx mori (Lepidoptera) is Putatively Involved in Response against BmNPV Infection. Wang, Xue-yang,Zhang, Shang-zhi,Yu, Dong,Ma, Yan,Fei, Dong-qiong,Yu, Hai-zhong,Xu, Jia-ping,Liu, Ming-hui. 2017

[7]TIP60 contributes to porcine embryonic development by regulating DNA damage response. Guo, Jing,Zhou, Wenjun,Niu, Ying-Jie,Shin, Kyung-Tae,Kim, Nam-Hyung,Cui, Xiang-Shun,Guo, Jing,Heo, Young Tae. 2018

[8]Envelope surface ultrastructure and specific gravity of artificially fertilized Pacific cod Gadus macrocephalus eggs. Bian, X.,Jin, X.,Wan, R.,Zhang, X.,Gao, T.,Sakurai, Y.,Yamamoto, J.. 2014

[9]Circular RNAs are abundant and dynamically expressed during embryonic muscle development in chickens. Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan. 2018

[10]Transcriptome analysis of gene expression patterns during embryonic development in golden cuttlefish (Sepia esculenta). Bian, Li,Liu, Changlin,Chen, Siqing,Zhao, Fazhen,Ge, Jianlong,Tan, Jie. 2018

[11]Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua. 2017

[12]First studies of embryonic and larval development of Coilia nasus (Engraulidae) under controlled conditions. Xu, Gangchun,Tang, Xue,Gu, Ruobo,Xu, Pao,Tang, Xue,Le, Guowei,Zhang, Chengxiang,Zheng, Jinliang. 2011

[13]A preliminary study of the reproductive biology of the biofouling organism Caprella acanthogaster (Crustacea, Amphipoda) in Sanggou Bay, China. Wei, Yanwei,Zhang, Jihong,Wu, Wenguang,Yao, Yongfeng,Fang, Jianguang,Chen, Jie.

[14]Potential Role of Cathepsin B in the Embryonic and Larval Development of Clam Meretrix meretrix. Wang, Xiaomei,Liu, Baozhong,Xiang, Jianhai,Wang, Xiaomei,Tang, Baojun.

[15]Histology and ultrastructure of the thymus during development in tilapia, Oreochromis niloticus. Cao, Jianmeng,Chen, Qiong,Lu, Maixin,Hu, Xinxin,Wang, Miao,Chen, Qiong,Hu, Xinxin.

[16]Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities. Fu, Bo,Liu, Di,Ma, Hong,Zhang, Dong-Jie,Guo, Zhen-Hua,Fu, Bo,Ma, Jian-Zhang,An, Tie-Zhu,Ren, Liang,Liu, Di,Yang, Xiu-Qin,Guo, Yun-Yun,Zhu, Meng,Bai, Jing.

[17]Transposable Element-Mediated Balancing Selection at Hsp90 Underlies Embryo Developmental Variation. Zhang, Bo,Xu, Lingling,Li, Qing,Xu, Yanan,Kang, Le,Jiang, Feng,Yang, Pengcheng,Kang, Le,Zhang, Bo.

[18]Functional characterization of a cystatin from the tick Rhipicephalus haemaphysaloides. Wang, Yujian,Zhou, Yongzhi,Gong, Haiyan,Cao, Jie,Zhang, Houshuang,Zhou, Jinlin,Wang, Yujian,Li, Xiangrui,Zhou, Jinlin. 2015

[19]Comparison of the developmental competence and quality of bovine embryos obtained by in vitro fertilization with sex-sorted and unsorted semen from seven bulls. Liu, Xia,Hu, Tingxi,Sun, Weijun,Hao, Haisheng,Liu, Yan,Zhao, Xueming,Zhu, Huabin,Du, Weihua,Hu, Tingxi.

[20]Effects of low temperatures on sexual reproduction of 'Tainong 1' mango (Mangifera indica). Huang, Jing-Hao,Liang, Guo-Lu,Wang, Wei-Xing,Huang, Jing-Hao,Cai, Zi-Jian,Wen, Shou-Xing,Huang, Jing-Hao,Ma, Wei-Hong,Zhang, Ling-Yuan. 2010

作者其他论文 更多>>

微信小程序