Development of SSR markers to study diversity in the genus Cymbidium

文献类型: 外文期刊

第一作者: Park, Yong-Jin

作者: Park, Yong-Jin;Chae, Soo-Cheon;Park, Ha Seung;Park, Pue Hee;Zhao, Weiguo;Moe, Kyaw Thu;Zhao, Weiguo;Song, Hong-Seon;Kim, You-Hyen;Chung, Jong-Wook;Cho, Young-Il;Park, Yong-Jin

作者机构:

关键词: simple sequence repeat marker: tetra-nucleotide;tri-nucleotide;di-nucleotide;genetic diversity;cross-amplification evaluation

期刊名称:BIOCHEMICAL SYSTEMATICS AND ECOLOGY ( 影响因子:1.381; 五年影响因子:1.351 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Cymbidium spp are important potted flowers with extremely high ornamental and economic value The present study reports the development of 14 new simple sequence repeat (SSR) markers through the construction of an enriched Cymbidium goeringii library and cross-amplification in Cymbidium sinensis and Cymbidium hybridium Of 525 322 (61 33%) clones had SSR motifs and among motifs di-nucleotides were predominant and followed by tri-nucleotide and tetra-nucleotide type In polymorphic analysis using 14 newly developed SSRs a total of 201 alleles across 96 Cymbidium accessions were detected with an average of 14 4 per locus The average heterozygosity was 0 394 The average gene diversity and polymorphism information content values were 0 394 and 0 639 respectively The mean genetic similarity coefficient was 0 4297 indicating a wide genetic variation among the Cymbidium accessions These newly developed SSRs will be useful tools for genotype identification germplasm conservation molecular breeding and assessments of genetic diversity and population structure in Cymbidium

分类号: Q5

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