Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos

文献类型： 外文期刊

第一作者： Liu Di

作者： Liu Di;Ma Hong;Wang Liang;Guo Zhen-hua;Li Zhong-qiu;Fu Bo;Liu Di;Fang Qing-chang;Ren Liang

作者机构：

关键词： EGFP;Histone deacetylation;Porcine;Somatic cell nuclear transfer;Trichostatin A

期刊名称：ZYGOTE （ 影响因子：1.442； 五年影响因子：1.536 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Transgenes integrated into mammalian cells are silenced rapidly. This phenomenon correlates with repressed chromatin structure marked by histone hypoacetylation. This study investigated the effect of trichostatin A (TSA; a histone-deacetylase inhibitor) on EGFP expression in transfected cells and embryonic development after somatic cell nuclear transfer (SCNT). Porcine adult fibroblasts were transfected with a pEGFP-C1 vector. Then transfected cells, donor cells for SCNT, were pretreated with TSA, with the untreated cells being used as the control. Expression of EGFP in donor cells and reconstructed embryos was detected when exposed to blue light. Results showed that the percentage of EGFP-positive cells significantly increased when the transfected cells were treated with TSA and the increased expression of EGFP was sustained to at least the morula stage. In addition, the cytotoxic effect of TSA on the transfected cells was dose dependent. In conclusion, TSA can rescue the silenced EGFP gene. Even after transferring the TSA-treated cells to enucleated recipient oocytes, TSA retained the ability to rescue a silenced EGFP gene. In addition, TSA had an impact on cell proliferation.

分类号： Q954.4

  • 相关文献

[1]Production of transgenic calves by somatic cell nuclear transfer. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Wang, LL,Wang, HP,Tang, B,Liu, Y,Li, R,Wan, R,Huang, YH,Li, N.

[2]Cloning and sequence analysis of oilseed rape (Brassica napus) SHP2 gene. Tan, Xiao-Li,Qi, Cun-Kou,Tan, Xiao-Li,Xia, Zong-Wei,Zhang, Li-Li,Zhang, Zhi-Yan,Guo, Zhong-Jian.

[3]Construction of Rice Site-Specific Chloroplast Transformation Vector and Transient Expression of EGFP Gene in Dunaliella Salina. Han, Xiaoxia,Zuo, Jia,Xie, Lingling,He, Ronghua,Gao, Jing,Cao, Mengliang,Yuan, Longping,Cao, Mengliang,Chang, Lan,Cao, Mengliang.

[4]Development of a GFP-expressing Ustilaginoidea virens strain to study fungal invasion and colonization in rice spikelets. Andargie, Mebeaselassie,Li, Luoye,Li, Jianxiong,Feng, Aiqing,Zhu, Xiaoyuan.

[5]Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test. Xue, Xianghong,Zheng, Xuexing,Liang, Hongru,Feng, Na,Zhao, Yongkun,Gao, Yuwei,Wang, Hualei,Yang, Songtao,Xia, Xianzhu,Xue, Xianghong,Yang, Songtao,Xia, Xianzhu,Zheng, Xuexing,Xia, Xianzhu. 2014

[6]A Preliminary Study on Stable Transfection of EGFP in Buffalo Cumulus Cells. Shang, Jiang-Hua,Yang, Chun-Yan,Qin, Jing,Zheng, Hai-Ying,Huang, Fen-Xiang,Wang, Jian,Liu, Hua-Zhong,Liu, Hua-Zhong. 2013

[7]Expression of Foreign Genes Demonstrates the Effectiveness of Pollen-Mediated Transformation in Zea mays. Yang, Liyan,Cui, Guimei,Wang, Yixue,Hao, Yaoshan,Du, Jianzhong,Wang, Changbiao,Zhang, Huanhuan,Wu, Shu-Biao,Sun, Yi,Yang, Liyan,Zhang, Hongmei,Wu, Shu-Biao,Sun, Yi. 2017

[8]Inactivation of eGFP DNA in PK-15 cells via CRISPR/Cas9 system. Ishag, Hassan Z. A.,Liu, Maojun,Yang, Ruosong,Xiong, Qiyan,Feng, Zhixin,Shao, Guoqing,Liu, Maojun. 2017

[9]Production of transgenic blastocyst by nuclear transfer from different types of somatic cells in cattle. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Wang, HP,Wang, LL,Fang, CG,Wan, R,Liu, Y,Li, R,Li, N.

[10]Treating Cloned Embryos, But Not Donor Cells, with 5-aza-2 '-deoxycytidine Enhances the Developmental Competence of Porcine Cloned Embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Liu, Shi Chao,Zhou, Yang,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,He, Hong Bin. 2013

[11]Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Li, Jing Yu,Xue, Bing Hua,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,Wu, Zhan Feng,Zhang, Ji Guang.

[12]Effect of Pyruvate and Lactic Acid on the Development of Porcine Embryos. Fu Bo,Llu Di,Ma Hong,Li Zhong-qiu,Wang Liang,Bai Jing. 2012

[13]Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer. Liu, Guoqian,Liu, Kai,Wei, Hengxi,Li, Li,Zhang, Shouquan,Liu, Guoqian,Liu, Kai,Wei, Hengxi,Li, Li,Zhang, Shouquan.

[14]Screening somatic cell nuclear transfer parameters for generation of transgenic cloned cattle with intragenomic integration of additional gene copies that encode bovine adipocyte-type fatty acid-binding protein (A-FABP). Guo, Yong,Sheng, Xihui,Chang, Di,Qi, Xiaolong,Wang, Xiangguo,Liu, Yunhai,Ni, Hemin,Li, Hejuan,Wang, Ying,Yan, Xingrong,Li, Junya.

[15]Multi-transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis. Kong, Siyuan,Ruan, Jinxue,Xin, Leilei,Fan, Junhua,Xia, Jihan,Liu, Zhiguo,Mu, Yulian,Yang, Shulin,Li, Kui.

[16]Using a nano-flare probe to detect RNA in live donor cells prior to somatic cell nuclear transfer. Fu, Bo,Ma, Jian-zhang,An, Tie-zhu,Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-hua,Ren, Liang,Liu, Di,Yang, Xiu-qin,Zhu, Meng,Bai, Jing.

[17]Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei. Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-Hua,Wang, Liang,Li, Zhong-Qiu,Peng, Fu-Gang,Liu, Di,Bai, Jing.

[18]Production of transgenic dairy goat expressing human alpha-lactalbumin by somatic cell nuclear transfer. Feng, Xiujing,Cao, Shaoxian,Wang, Huili,Meng, Chunhua,Li, Jingxin,Jiang, Jin,Qian, Yong,Su, Lei,He, Qiang,Zhang, Qingxiao,Feng, Xiujing,Li, Jingxin,Jiang, Jin,Su, Lei,Zhang, Qingxiao,Feng, Xiujing,Feng, Xiujing,Cao, Shaoxian,Wang, Huili,Meng, Chunhua,Qian, Yong.

[19]Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig. Hua, Z.,Liu, X.,Bi, Y.,Xiao, H.,Hua, W.,Li, L.,Zhang, L.,Ren, H.,Zheng, X.,Xu, G..

[20]Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos. Huan, Yan Jun,Wang, Hong Mei,Bin He, Hong,Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Li, Jing Yu,Kong, Qing Ran,Liu, Zhong Hua,Zhu, Jiang,Wu, Zhan Feng,Zhang, Ji Guang.

作者其他论文 更多>>

微信小程序