Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos

文献类型： 外文期刊

第一作者： Liu Di

作者： Liu Di;Ma Hong;Wang Liang;Guo Zhen-hua;Li Zhong-qiu;Fu Bo;Liu Di;Fang Qing-chang;Ren Liang

作者机构：

关键词： EGFP;Histone deacetylation;Porcine;Somatic cell nuclear transfer;Trichostatin A

期刊名称：ZYGOTE （ 影响因子：1.442； 五年影响因子：1.536 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Transgenes integrated into mammalian cells are silenced rapidly. This phenomenon correlates with repressed chromatin structure marked by histone hypoacetylation. This study investigated the effect of trichostatin A (TSA; a histone-deacetylase inhibitor) on EGFP expression in transfected cells and embryonic development after somatic cell nuclear transfer (SCNT). Porcine adult fibroblasts were transfected with a pEGFP-C1 vector. Then transfected cells, donor cells for SCNT, were pretreated with TSA, with the untreated cells being used as the control. Expression of EGFP in donor cells and reconstructed embryos was detected when exposed to blue light. Results showed that the percentage of EGFP-positive cells significantly increased when the transfected cells were treated with TSA and the increased expression of EGFP was sustained to at least the morula stage. In addition, the cytotoxic effect of TSA on the transfected cells was dose dependent. In conclusion, TSA can rescue the silenced EGFP gene. Even after transferring the TSA-treated cells to enucleated recipient oocytes, TSA retained the ability to rescue a silenced EGFP gene. In addition, TSA had an impact on cell proliferation.

分类号： Q954.4

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