Large-scale analysis of phosphorylated proteins in maize leaf

文献类型： 外文期刊

第一作者： Bi, Ying-Dong

作者： Bi, Ying-Dong;Lu, Tian-Cong;Shen, Zhuo;Chen, Yi-Bo;Wang, Bai-Chen;Bi, Ying-Dong;Lu, Tian-Cong;Shen, Zhuo;Chen, Yi-Bo;Wang, Bai-Chen;Bi, Ying-Dong;Wang, Hong-Xia;Li, Xiao-hui

作者机构：

关键词： Maize;NanoLC-MS/MS;Phosphorylation

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

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年卷期：

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收录情况： SCI

摘要： Phosphorylation is an ubiquitous regulatory mechanism governing the activity, subcellular localization, and intermolecular interactions of proteins. To identify a broad range of phosphoproteins from Zea mays, we enriched phosphopeptides from Zea mays leaves using titanium dioxide microcolumns and then extensively fractionated and identified the phosphopeptides by mass spectrometry. A total of 165 unique phosphorylation sites with a putative role in biological processes were identified in 125 phosphoproteins. Most of these proteins are involved in metabolism, including carbohydrate and protein metabolism. We identified novel phosphorylation sites on translation initiation factors, splicing factors, nucleolar RNA helicases, and chromatin-remodeling proteins such as histone deacetylases. Intriguingly, we also identified phosphorylation sites on several proteins associated with photosynthesis, and we speculate that these sites may be involved in carbohydrate metabolism or electron transport. Among these phosphoproteins, phosphoenolpyruvate carboxylase and NADH: nitrate reductase (NR) which catalyzes the rate-limiting and regulated step in the pathway of inorganic nitrogen assimilation were identified. A conserved phosphorylation site was found in the cytochrome b5 heme-binding domain of NADH: nitrate reductase, suggesting that NADH: nitrate reductase is phosphorylated by the same protein kinase or highly related kinases. These data demonstrate that the pathways that regulate diverse processes in plants are major targets of phosphorylation.

分类号： Q94

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