CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B1 Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

文献类型： 外文期刊

第一作者： Wang, Zhilong

作者： Wang, Zhilong;Wei, Luyu;Chen, Yiping;Ruan, Shilong;Chen, Yiping;Chen, Yiping

作者机构： Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China;Daye Publ Inspection & Test Ctr, Daye 435100, Hubei, Peoples R China;Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Guangdong, Peoples R China;Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr,Genome Anal Lab M, Shenzhen 518120, Guangdong, Peoples R China

关键词： mycotoxin; CRISPR; Cas system; aptamer; horseradish peroxidase; versatile signal reporter

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 2022影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 10 期

页码：

收录情况： SCI

摘要： Herein, we report a CRISPR/Cas12a-assisted chemiluminescence sensor for aflatoxin B1 (AFB1) detection based on functional nucleic-acid-mediated target recognition and in-pipet rolling circle amplification-mediated signal amplification. In this sensor, we performed rolling circle amplification on the inside of the pipet to enrich horseradish peroxidase (pipet-poly-HRP). When AFB1 is present, it interacts with functional nucleic acids and results in the release of the activator. The activator is designed to activate the CRISPR/Cas12a system, which cleaves the pipet-poly-HRP to liberate HRP. The freed HRP can then be measured by chemiluminescence to quantify AFB1. This CRISPR/Cas12a-assisted chemiluminescence sensor enables facile, highly sensitive, and specific detection of AFB1 , with a linear range from 50 pg/mL to 100 ng/mL and a detection limit of 5.2 pg/mL. Furthermore, it exhibits satisfactory recovery and has successfully challenged AFB1 detection in cereal samples. The proposed sensor offers a novel rapid screening approach that holds great promise for food security monitoring.

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