OmpA is a virulence factor of Riemerella anatipestifer

文献类型： 外文期刊

第一作者： Hu, Qinghai

作者： Hu, Qinghai;Han, Xiangan;Zhou, Xiaojin;Ding, Chan;Zhu, Yinyu;Yu, Shengqing

作者机构：

关键词： adhesin;OmpA

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Riemerella anatipestifer infection is probably the most economically important disease of farm ducks worldwide. The pathogen R. anatipestifer causes septicemia anserum exsudativa in ducks, but little is known about the molecular basis of its pathogenesis and the virulence factors involved. In this study, by deleting ompA gene from R. anatipestifer serotype 2 strain Th4, we constructed a mutant strain Th4 Delta ompA to investigate whether R. anatipestifer OmpA is an important virulence factor. Results showed that although the growth curve, bacterial and colony morphology of Th4 Delta ompA in tryptic soybean broth (TSB) or on TSB agar were similar to its parent strain Th4, the adhesion and invasion capacities of mutant strain to Vero cells were decreased significantly. Furthermore, the median lethal dose (LD50) of both strains was determined to measure the virulence with 10-day-old Cherry Valley ducklings. The results showed that LD50 of Th4 Delta ompA mutant was > 10(10) colony forming units (CFU), it was attenuated significantly in comparison with that of Th4 which LD50 was 4.41 x 10(8) CFU. Additional analysis indicated that blood bacterial loading of ducklings infected with the Th4 Delta ompA mutant were much lower than those of Th4-infected ducklings. The results demonstrate that OmpA is a virulence factor of R. anatipestifer, and that it may act as an adhesin

分类号： S8

  • 相关文献

[1]Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs. Yang, Feng,Li, Jing,Zhang, Jilei,Wang, Yaoyao,Wang, Jiawei,Zhou, Xin,Wang, Chengming,Jelocnik, Martina,Polkinghorne, Adam,Gong, Jianseng,Kaltenboeck, Bernhard,Wang, Chengming,Feng, Zhixin,Pannekoek, Yvonne,Borel, Nicole,Song, Chunlian,Jiang, Ping. 2017

[2]Characterization of roles of SpaA in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells. Zhu, Weifeng,Cai, Chengzhi,Wang, Ya,Li, Jingtao,Kang, Chao,Sun, Xiaomei,Jin, Meilin,Zhu, Weifeng,Cai, Chengzhi,Wang, Ya,Li, Jingtao,Kang, Chao,Sun, Xiaomei,Jin, Meilin,Wu, Chao,Sun, Xiaomei,Jin, Meilin,Wu, Chao,Sun, Xiaomei,Jin, Meilin,Zhu, Weifeng. 2017

作者其他论文 更多>>

微信小程序