Electron Tomography Reveals Polyhedrin Binding and Existence of both Empty and Full Cytoplasmic Polyhedrosis Virus Particles inside Infectious Polyhedra

文献类型： 外文期刊

第一作者： Chen, Justin

作者： Chen, Justin;Zhou, Z. Hong;Chen, Justin;Atanasov, Ivo;Ryazantsev, Sergey;Zhou, Z. Hong;Sun, Jingchen;Ryazantsev, Sergey

作者机构：

关键词： RNA;viral capsid;viral particles;foreign proteins;polyhedrin: B-spike;A-spike;RNA encapsidation;infectious polyhedra;viral protein packaging

期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.103； 五年影响因子：5.078 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Previous studies have described the structure of purified cytoplasmic polyhedrosis virus (CPV) and that of polyhedrin protein. However, how polyhedrin molecules embed CPV particles inside infectious polyhedra is not known. By using electron tomography, we show that CPV particles are occluded within the polyhedrin crystalline lattice with a random spatial distribution and interact with the polyhedrin protein through the A-spike rather than as previously thought through the B-spike. Furthermore, both full (with RNA) and empty (no RNA) capsids were found inside polyhedra, suggesting a spontaneous RNA encapsidating process for CPV assembly in vivo.

分类号： Q934.2

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