Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis

文献类型： 外文期刊

第一作者： Li, Zhao

作者： Li, Zhao;Zhang, Zengyan;Du, Lipu;Xu, Huijun;Xin, Zhiyong;Li, Zhao;Zhou, Miaoping;Ren, Lijuan;Zhang, Boqiao

作者机构：

关键词： Defensin RsAFP2;Fusarium graminearum;Resistance;Rhizoctonia cerealis;Transgenic wheat;Triticum aestivum

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Fusarium head blight (scab), primarily caused by Fusarium graminearum, is a devastating disease of wheat (Triticum aestivum L.) worldwide. Wheat sharp eyespot, mainly caused by Rhizoctonia cerealis, is one of the major diseases of wheat in China. The defensin RsAFP2, a small cyteine-rich antifungal protein from radish (Raphanus sativus), was shown to inhibit growth in vitro of agronomically important fungal pathogens, such as F. graminearum and R. cerealis. The RsAFP2 gene was transformed into Chinese wheat variety Yangmai 12 via biolistic bombardment to assess the effectiveness of the defensin in protecting wheat from the fungal pathogens in multiple locations and years. The genomic PCR and Southern blot analyses indicated that RsAFP2 was integrated into the genomes of the transgenic wheat lines and heritable. RT-PCR and Western blot proved that the RsAFP2 was expressed in these transgenic wheat lines. Disease tests showed that four RsAFP2 transgenic lines (RA1-RA4) displayed enhanced resistance to F. graminearum compared to the untransformed Yangmai 12 and the null-segregated plants. Assays on Q-RT-PCR and disease severity showed that the express level of RsAFP2 was associated with the enhanced resistance degree. Two of these transgenic lines (RA1 and RA2) also exhibited enhanced resistance to R. cerealis. These results indicated that the expression of RsAFP2 conferred increased resistance to F. graminearum and R. cerealis in transgenic wheat.

分类号： Q78

  • 相关文献

[1]The wheat AGC kinase TaAGC1 is a positive contributor to host resistance to the necrotrophic pathogen Rhizoctonia cerealis. Zhu, Xiuliang,Yang, Kun,Wei, Xuening,Rong, Wei,Du, Lipu,Ye, Xingguo,Qi, Lin,Zhang, Zengyan,Zhang, Qiaofeng.

[2]The wheat NB-LRR gene TaRCR1 is required for host defence response to the necrotrophic fungal pathogen Rhizoctonia cerealis. Zhu, Xiuliang,Du, Lipu,Ye, Xingguo,Liu, Xin,Zhang, Zengyan,Lu, Chungui,Coules, Anne.

[3]Ecological fitness of fludioxonil-resistant Rhizoctonia cerealis strain and its cross-resistance to DMIs and validamycin. Xia, Xiaoming,Zhao, Ming,Wang, Hongyan,Ma, Hui,Wang, Kaiyun. 2012

[4]Identification and antifungal assay of a wheat beta-1,3-glucanase. Liu, Baoye,Lu, Yan,Xin, Zhiyong,Zhang, Zengyan. 2009

[5]Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat. Kong, XY,Gu, YQ,You, FM,Dubcovsky, J,Anderson, OD.

[6]A rice stress-responsive NAC gene enhances tolerance of transgenic wheat to drought and salt stresses. Saad, Abu Sefyan I.,Li, Xu,Li, He-Ping,Huang, Tao,Gao, Chun-Sheng,Guo, Mao-Wei,Cheng, Wei,Liao, Yu-Cai,Li, He-Ping,Gao, Chun-Sheng,Liao, Yu-Cai,Zhao, Guang-Yao.

[7]Expression of a potato antimicrobial peptide SN1 increases resistance to take-all pathogen Gaeumannomyces graminis var. tritici in transgenic wheat. Rong, Wei,Qi, Lin,Wang, Jingfen,Du, Lipu,Xu, Huijun,Zhang, Zengyan,Rong, Wei,Wang, Aiyun. 2013

[8]Overexpression of TaPIEP1, a pathogen-induced ERF gene of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana. Dong, Na,Liu, Xin,Lu, Yan,Du, LiPu,Xu, Huijun,Liu, Hongxia,Xin, Zhiyong,Zhang, Zengyan.

[9]Effects of plant height on type I and type II resistance to fusarium head blight in wheat. Yan, W.,Li, H. B.,Liu, C. J.,Yan, W.,Cai, S. B.,Ma, H. X.,Rebetzke, G. J.,Liu, C. J..

[10]Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1. Long, XiangYu,Balcerzak, Margaret,Gulden, Sigrun,Cao, Wenguang,Fedak, George,Ouellet, Therese,Long, XiangYu,Wei, Yu-Ming,Zheng, You-Liang,Somers, Daryl.

[11]A Wheat Cinnamyl Alcohol Dehydrogenase TaCAD12 Contributes to Host Resistance to the Sharp Eyespot Disease. Rong, Wei,Luo, Meiying,Shan, Tianlei,Wei, Xuening,Du, Lipu,Xu, Huijun,Zhang, Zengyan. 2016

[12]Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR. Guo, Yingpeng,Li, Wei,Sun, Haiyan,Wang, Ning,Chen, Huaigu,Guo, Yingpeng,Yu, Hanshou. 2012

[13]Quantitative trait loci for resistance to Sharp Eyespot (Rhizoctonia cerealis) in recombinant inbred wheat lines from the cross Niavt 14 x Xuzhou 25. Jiang, Yanjie,Zhu, Fangfang,Cai, Shibin,Wu, Jizhong,Zhang, Qiaofeng. 2016

[14]Characterization of Rhizoctonia cerealis sensitivity to thifluzamide in China. Sun, Haiyan,Wang, Chengfeng,Li, Wei,Zhang, Aixiang,Deng, Yuanyu,Chen, Huaigu.

[15]Changes in Activities of Antioxidant-Related Enzymes in Leaves of Resistant and Susceptible Wheat Inoculated with Rhizoctonia cerealis. Liu Hong-xia,Xin Zhi-yong,Zhang Zeng-yan. 2011

[16]Homozygous and heterozygous point mutations in succinate dehydrogenase subunits b, c and d of Rhizoctonia cerealis conferring resistance to thifluzamide. Sun, Hai-Yan,Lu, Chao-Qun,Li, Wei,Deng, Yuan-Yu,Chen, Huai-Gu.

[17]The escalating threat of Rhizoctonia cerealis, the causal agent of sharp eyespot In wheat. Hamada, Mohamed Sobhy,Yin, Yanni,Ma, Zhonghua,Chen, Huaigu.

[18]Two members of TaRLK family confer powdery mildew resistance in common wheat. Tingting Chen,Jin Xiao,Jun Xu,Wentao Wan,Bi Qin,Aizhong Cao,Wei Chen,Liping Xing,Chen Du,Xiquan Gao,Shouzhong Zhang,Ruiqi Zhang,Wenbiao Shen,Haiyan Wang,Xiue Wang. 2016

[19]RNA-seq analysis of unintended effects in transgenic wheat overexpressing the transcription factor GmDREB1. Jiang, Qiyan,Niu, Fengjuan,Sun, Xianjun,Hu, Zheng,Li, Xinhai,Ma, Youzhi,Zhang, Hui. 2017

[20]Expression of the intact C-4 type pepc gene cloned from maize in transgenic winter wheat. Chen, XQ,Zhang, XD,Liang, RQ,Zhang, LQ,Yang, FP,Cao, MQ. 2004

作者其他论文 更多>>

微信小程序