Double synergic chitosan-coated poly (lactic-co-glycolic) acid nanospheres loaded with nucleic acids as an intranasally administered vaccine delivery system to control the infection of foot-and-mouth disease virus

文献类型： 外文期刊

第一作者： Li, Xian

作者： Li, Xian;Zhang, Zhong-wang;Zhang, Fu-dong;Li, Jia-hao;Zhai, Kai-ge;Liu, Xin-sheng;Pan, Li;Li, Xian;Zhang, Zhong-wang;Zhang, Fu-dong;Li, Jia-hao;Zhai, Kai-ge;Liu, Xin-sheng;Pan, Li;Liu, Xin-sheng;Pan, Li

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关键词： FMDV; Chitosan delivery system; Fms-like tyrosine kinase receptor 3 ligand; Nasal mucosal vaccine; Cross-protective

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：7.6； 五年影响因子：5.8 ）

ISSN： 0166-3542

年卷期： 2024 年 226 卷

页码：

收录情况： SCI

摘要： Background & aims: The spread of foot-and-mouth disease virus (FMDV) through aerosol droplets among clovenhoofed ungulates in close contact is a major obstacle for successful animal husbandry. Therefore, the development of suitable mucosal vaccines, especially nasal vaccines, to block the virus at the initial site of infection is crucial. Patients and methods: Here, we constructed eukaryotic expression plasmids containing the T and B-cell epitopes (pTB) of FMDV in tandem with the molecular mucosal adjuvant Fms-like tyrosine kinase receptor 3 ligand (Flt3 ligand, FL) (pTB-FL). Then, the constructed plasmid was electrostatically attached to mannose-modified chitosan-coated poly(lactic-co-glycolic) acid (PLGA) nanospheres (MCS-PLGA-NPs) to obtain an active nasal vaccine targeting the mannose-receptor on the surface of antigen-presenting cells (APCs). Results: The MCS-PLGA-NPs loaded with pTB-FL not only induced a local mucosal immune response, but also induced a systemic immune response in mice. More importantly, the nasal vaccine afforded an 80% protection rate against a highly virulent FMDV strain (AF72) when it was subcutaneously injected into the soles of the feet of guinea pigs. Conclusions: The nasal vaccine prepared in this study can effectively induce a cross-protective immune response against the challenge with FMDV of same serotype in animals and is promising as a potential FMDV vaccine.

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