Molecular analysis of the QM gene from Penaeus monodon and its expression on the different ovarian stages of development

文献类型： 外文期刊

第一作者： Zhou, FaLin

作者： Zhou, FaLin;Jiang, ShiGui;Huang, JianHua;Qiu, Lihua;Zhang, Dianchang;Su, Tiannfeng;Zhou, FaLin

作者机构：

关键词： Molecular analysis;QM;mRNA expression;Penaeus monodon

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： In present study, a QM gene was obtained from the ovary and neurosecretory organ in eyestalk cDNA library of black tiger prawn (Penaeus monodon). The full-length black tiger prawn QM (PmQM) cDNA contained a 5'-UTR of 41bp, an ORF of 663bp encoding a polypeptide of 220 amino acids with molecular weight 25.5kDa, and a 3'-UTR of 54bp. Homology analysis of the deduced amino acid sequence of the PmQM with other known QM sequences by MatGAT software revealed that the PmQM was high homology with other invertebrates. A conserved signature sequence of the QM family was found in the PmQM deduced amino acid sequence. Analysis of the tissue expression pattern of the PmQM gene showed that the PmQM mRNA was expressed in all tissues tested, with highest levels in ovary. Furthermore, the PmQM expression was found to be different in three important ovarian stages of development. The results indicated PmQM might play an important role in ovarian development.

分类号： Q7

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