Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus

文献类型： 外文期刊

第一作者： Ge, Jun-Qing

作者： Ge, Jun-Qing;Xu, Yi-Peng;Zhang, Chuan-Xi;Ge, Jun-Qing;Xu, Yi-Peng;Zhang, Chuan-Xi;Ge, Jun-Qing;Gao, Guo-Hui

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关键词： Bombyx mori nucleopolyhedrovirus;Bm75;P33;Late gene;Nonstructural protein

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Open reading frame 75 (Bm-p33) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a homologue of Autographa californica multiple nucleopolyhedrovirus ORF92. The gene is conserved among all baculoviruses that have been completely sequenced to date and is considered to be a baculovirus core set gene. No amino acid mutation was found in Bm-p33 sequences among six BmNPV strains differing in geography, phenotype, or host. The Bm-p33 transcript can be detected as early as 12h post infection (h p.i.) and remains detectable until 96h p.i. The Bm-p33 protein was detected in cell lysates from 18h p.i. through 96h p.i., and no positive band could be detected in budded viruses (BVs) and occlusion-derived viruses (ODVs) by western blot using anti-Bm-p33 serum. Immunofluorescence microscopy indicated that Bm-p33 accumulated in the nuclear membrane and the intranuclear region, especially near the nuclear membrane of the virus-infected cells. Bm75 RNAi significantly decreased the mRNA level. However, no obvious effects on ODV formation and BV production in BmNPV-infected cells could be detected. Bm-p33 is a BmNPV late gene encoding a nonstructural protein which may function mainly in the nucleus of the infected cells.

分类号： Q7

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