Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes

文献类型： 外文期刊

第一作者： Zhong, Ruichun

作者： Zhong, Ruichun;Han, Zhuqiang;Jiang, Jing;He, Liangqiong;Tang, Ronghua;Xiong, Faqian;Han, Zhuqiang;Jiang, Jing;Tang, Ronghua;Zhuang, Weijian

作者机构：

关键词： DNA: polymorphism;genotype;genetic relationship;functional genetic variation

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Cultivated peanut possesses an extremely narrow genetic basis. Polymorphism is considerably difficult to identify with the use of conventional biochemical and molecular tools. For the purpose of obtaining considerable DNA polymorphisms and fingerprinting cultivated peanut genotypes in a convenient manner, start codon targeted polymorphism technique was used to study genetic diversity and relatedness among 20 accessions of four major botanical varieties of peanut. Of 36 primers screened, 18 primers could produce unambiguous and reproducible bands. All 18 primers generated a total of 157 fragments, with a mean of 8.72 ranging from 4 to 17 per primer. Of 157 bands, 60 (38.22%) were polymorphic. One to seven polymorphic bands were amplified per primer, with 3.33 polymorphic bands on average. Polymorphism per primer ranged from 14.29 to 66.67%, with an average of 36.76%. The results revealed that not all accessions of the same variety were grouped together and high genetic similarity was detected among the tested genotypes based on cluster analysis and genetic distance analysis, respectively. Further, accession-specific markers were observed in several accessions. All these results demonstrated the following: (1) start codon targeted polymorphism technique can be utilized to identify DNA polymorphisms and fingerprint cultivars in domesticated peanut, and (2) it possesses considerable potential for studying genetic diversity and relationships among peanut accessions.

分类号： Q7

  • 相关文献

[1]A core collection and mini core collection of Oryza sativa L. in China. Zhang, Hongliang,Zhang, Dongling,Wang, Meixing,Sun, Junli,Qi, Yongwen,Li, Jinjie,Li, Zichao,Wei, Xinghua,Tang, Shengxiang,Han, Longzhi,Qiu, Zongen.

[2]Study on the Genetic Relationship of Panax Notoginseng and Its Wild Relatives Based on Fourier Translation Infrared Spectroscopy. Li Yun,Zhang Jin-yu,Xu Fu-rong,Li Yun,Wang Yuan-zhong,Yang Wei-ze,Yang Shao-bing,Zhang Jin-yu. 2016

[3]Diversity, population structure, and evolution of local peach cultivars in China identified by simple sequence repeats. Shen, Z. J.,Zhang, Z.,Shen, Z. J.,Ma, R. J.,Cai, Z. X.,Yu, M. L.. 2015

[4]The relationships among Cymbidium sinense cultivars based on RAPD analysis. Zhu, Genfa,Li, Dongmei,Li, Dongmei,Ye, Qingsheng,Guo, Zhenfei. 2008

[5]GENETIC RELATIONSHIPS OF CHINESE GRAPE ACCESSION TO EUROPEAN AND AMERICAN CULTIVARS ASSESSED BY MICROSATELLITE MARKERS. Guo, D. L.,Zhang, J. Y.,Zhang, G. H.,Li, M.,Liu, C. H.. 2010

[6]Infraspecific Classification of Ziziphus jujuba Mill. Using AFLP Marker Technique. Bai, R. X.,Peng, J. Y.,Li, L.,Zhang, Y.,Li, X. Y.. 2009

[7]The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository. Chen, L,Gao, QK,Chen, DM,Xu, CJ.

[8]Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA. Meng, Xian Hong,Wang, Qing Yin,Liu, Ping,Kong, Jie,Meng, Xian Hong,Jang, In Kwon.

[9]Evaluation of genetic diversity and relationships in orchardgrass (Dactylis glomerata L.) germplasm based on SRAP markers. Zhang, Xin-Quan,Zeng, Bing,Lan, Ying,Yang, Wu-Yun.

[10]Molecular characterization and genetic diversity of different genotypes of Oryza sativa and Oryza glaberrima. Chen, Caijin,He, Wenchuang,Nassirou, Tondi Yacouba,Nsabiyumva, Athanase,Dong, Xilong,Jin, Deming,Adedze, Yawo Mawunyo Nevame. 2017

[11]Genetic variability and relationships between and within grape cultivated varieties and wild species based on SRAP markers. Guo, Dalong,Zhang, Junyu,Zhang, Guohai,Li, Meng,Zhang, Qian,Liu, Chonghuai. 2012

[12]High gene flows promote close genetic relationship among fine wool sheep populations (Ovis aries) in China. Han Ji-long,Guo Ting-ting,Liu Jian-bin,Niu Chun-e,Yuan Chao,Yue Yao-jing,Yang Bo-hui,Yang Min. 2016

[13]Genetic diversity and similarity of pear (Pyrus L.) cultivars native to East Asia revealed by SSR (simple sequence repeat) markers. Bao, Lu,Chen, Kunsong,Zhang, Dong,Cao, Yufen,Yamamoto, Toshiya,Teng, Yuanwen. 2007

[14]Genetic relationship between grain yield and the contents of protein and fat in a recombinant inbred population of rice. Yu, Yong-Hong,Li, Gang,Fan, Ye-Yang,Zhang, Ke-Qin,Min, Jie,Zhu, Zhi-Wei,Zhuang, Jie-Yun.

[15]Evaluation of the genetic diversity and population structure of Chinese indigenous horse breeds using 27 microsatellite markers. Ling, Y. H.,Ma, Y. H.,Guan, W. J.,Cheng, Y. J.,Wang, Y. P.,Han, J. L.,Zhao, Q. J.,He, X. H.,Pu, Y. B.,Fu, B. L.,Ling, Y. H.,Ma, Y. H.,Guan, W. J.,Cheng, Y. J.,Wang, Y. P.,Han, J. L.,Zhao, Q. J.,He, X. H.,Pu, Y. B.,Fu, B. L.,Mang, L.. 2011

[16]Evaluation of genetic diversity, population structure, and linkage disequilibrium among elite Chinese wheat (Triticum aestivum L.) cultivars. Guo, Xiaomin,Gao, Ainong,Liu, Weihua,Yang, Xinming,Li, Xiuquan,Li, Lihui. 2011

[17]Genetic Dissection of Root Morphological Traits Related to Nitrogen Use Efficiency in Brassica napus L. under Two Contrasting Nitrogen Conditions. Wang, Jie,Dun, Xiaoling,Shi, Jiaqin,Wang, Xinfa,Liu, Guihua,Wang, Hanzhong. 2017

[18]Population genetics of Ageratina adenophora using inter-simple sequence repeat (ISSR) molecular markers in China. Wan, Fang-Hao,Guo, Jian-Ying,Gui, Fu-Rong,Gui, Fu-Rong,Wan, Fang-Hao,Guo, Jian-Ying. 2008

[19]Analysis of Genetic Diversity in Banana Cultivars (Musa spp.) Using Sequence-Related Amplified Polymorphism Markers. Wei, J. Y.,Wei, S. X.,Xie, Z. S.,Xu, G. Y.,Chen, Y. Y.. 2011

[20]Genetic Diversity of Banana Cultivars (Musa spp.) Using SRAP Markers. Wei, Junya,Wei, Shouxing,Chen, Yeyuan. 2009

作者其他论文 更多>>

微信小程序