Discrimination between Haemaphysalis longicornis and H. qinghaiensis based on the partial 16S rDNA and the second internal transcribed spacer (ITS-2)

文献类型： 外文期刊

第一作者： Tian, Zhancheng

作者： Tian, Zhancheng;Liu, Guangyuan;Xie, Junren;Yin, Hong;Luo, Jianxun;Zhang, Liyan;Zhang, Ping;Luo, Jin

作者机构：

关键词： nucleotide;16S rDNA: 16S ribosomal DNA;genetic marker;hypervariable region;internal transcribed spacer-2: ITS-2;genetic marker;sequence variation;GenBank sequence data;population genetic structure

期刊名称：EXPERIMENTAL AND APPLIED ACAROLOGY （ 影响因子：2.132； 五年影响因子：2.196 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the present study, two hard tick species, Haemaphysalis longicornis and H. qinghaiensis from North-western China were characterized genetically by the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA and partial 16S rDNA. Based on a fragment within the hypervariable region of 16S rDNA with the length of approximately 453 bp, the phylogenetic trees were constructed by Neighbor-Joining and Maximum-parsimony methods. The results indicated that the phylogenetic status of H. qinghaiensis was distant from that of H. longicornis and closer to H. flava. Furthermore, the ITS-2 rDNA was amplified by PCR and sequenced from individual ticks. The length of ITS-2 is 1,606 bp for H. longicornis and 1,162 bp for H. qinghaiensis. Although sequence variation between the immature stages of H. longicornis was 0.1-0.4%, nucleotide differences between the tested species ranged 2.1-23.2%, indicating that ITS-2 rDNA sequences are genetic markers for the differentiation of the two hard ticks in China. Hence, a PCR-linked restriction fragment length polymorphism (RFLP) approach was developed for their unequivocal differentiation based on ITS-2 rDNA, which provides the foundation for further studies on ticks in China and has implications for studying the population genetic structure of the ticks and for identification and differentiation of closely related ticks.

分类号： Q959

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