Using double-stranded RNA to explore the role of heat shock protein genes in heat tolerance in Bemisia tabaci (Gennadius)

文献类型： 外文期刊

第一作者： Lue, Zhi-Chuang

作者： Lue, Zhi-Chuang;Wan, Fang-Hao;Wan, Fang-Hao

作者机构：

关键词： double-stranded RNA;mRNA transcript: expression;gene function;heat tolerance

期刊名称：JOURNAL OF EXPERIMENTAL BIOLOGY （ 影响因子：3.312； 五年影响因子：3.828 ）

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收录情况： SCI

摘要： The whitefly, Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) biotype B, is one of the most destructive invasive pests of field and glasshouse crops, and has a high tolerance to heat. Our previous work found that whitefly females are more heat tolerant than males. In the present study, real-time PCR and double-stranded RNA (dsRNA) methods were used to explore the role of heat shock protein (Hsp) genes in whitefly of both sexes; this provided further evidence of the mechanism underlying the differential heat tolerance abilities of females and males. The results showed that both hsp23 and hsp70 mRNA expression levels were higher in females than in males from 37.5 to 42 degrees C, while at the extreme temperature of 44 degrees C the hsp70 mRNA level was higher in males than in females. There was no significant difference in hsp90 mRNA expression between females and males under heat shock conditions. Furthermore, the survival rate of females fed hsp23 or hsp70 dsRNA significantly decreased following heat shock at 44 degrees C for 1h, but male survival rate was not significantly affected. Additionally, the survival rate of both females and males showed no significant change after they were fed with hsp90 dsRNA. Collectively, the present study shows that the optimum mRNA expression of Hsp genes in females promotes a higher survival rate under heat shock conditions; hsp23 and hsp70 play a key role for heat tolerance in females but not in males, and hsp90 shows no significant role in heat tolerance in either females or males. Further, our study indicates that feeding with dsRNA is an effective method by which to study gene function, and the simplicity of this approach opens the way for further research on gene function in different sexes and diverse groups of species.

分类号： Q-33

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