Characterization of the Transgenic Rice Event TT51-1 and Construction of a Reference Plasmid

文献类型： 外文期刊

第一作者： Cao, Yinglong

作者： Cao, Yinglong;Wu, Gang;Wu, Yuhua;Nie, Shujing;Zhang, Li;Lu, Changming

作者机构：

关键词： TT51-1;characterization;rearrangement;reference plasmid;calibrator

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： Transgenic rice TT51-1 (BT63) is an insect resistant strain that was granted for safety certificate in China in 2009. This study characterizes the transgenic event TT51-1 using a GenomeWalker strategy. The organization of the transgenes indicated that the transgenes Oh two plasmids, pFHBTl and pGL2RC7, had been integrated at the same locus. The sequence of the event TT51-1 spanned 8725 bp, including a truncated CrylAb/Ac cassette, an intact CrylAb/Ac cassette, two Amp gene segments, and an Hph gene segment. The 5' and 3' plant flanking sequences were isolated and used to locate the transgenes to chromosome 10 in TT51-1. The isolated TT51 -1 fragment and a fragment of the rice PLD gene were integrated into a plasmid vector, to create plasmid pK-TTS 1 as a calibrator for detecting rice containing TT51 - 1. Analysis of unknown samples indicated that the reference plasmid was a reliable alternative to TT51- 1 genomic DNA.

分类号： R15

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