Ionic liquid-based ultrasonic/microwave-assisted extraction combined with UPLC for the determination of anthraquinones in rhubarb
文献类型: 外文期刊
第一作者: Lu, Chunxia
作者: Lu, Chunxia;Wang, Hongxin;Lv, Wenping;Ma, Chaoyang;Xu, Pao;Zhu, Jian;Xie, Jun;Liu, Bo;Zhou, Qunlan
作者机构:
关键词: Anthraquinones;Ionic liquid;Rhubarb;Ultra performance liquid chromatography;Ultrasonic/microwave-assisted extraction
期刊名称:CHROMATOGRAPHIA ( 影响因子:2.044; 五年影响因子:1.725 )
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收录情况: SCI
摘要: Ionic liquid-based ultrasonic/microwave-assisted extraction (IL-UMAE) of five anthraquinones (physcion, chrysophanol, emodin, rhein, and aloe-emodin) from rhubarb was first studied. Several parameters of UMAE were optimized, and the results were compared with of the heat-reflux extraction (HRE), ultrasound, and microwave-assisted extraction (MAE and UAE). The optimal UMAE conditions were as follows: the solvent was 2.0 mol L~(-1) 1-butyl-3- methylimidazolium bromide [bmim]Br solution, the ration of solid/liquid (g mL~(-1)) was 1:15, time was 2 min, and microwave power was 500 W. Under these UMAE conditions, total content of five anthraquinones was 28.00 mg g ~(-1). Compared with the conventional HRE, regular MAE and UAE techniques, the proposed approach exhibited higher efficiency (18.90-24.40% enhanced) and shorter extraction time (from 6 h to 2 min). The anthraquinones were then determined by ultra performance liquid chromatography (UPLC). Based on optimized conditions, contents of physcion, chrysophanol, emodin, rhein and aloe-emodin in rhubarb collected from different cultivated areas were 0.68-2.99, 5.03-15.40, 0.48-4.34, 0.025-3.93 and 0.26-2.56 mg g~(-1), respectively. This study suggests that IL-UMAE was an efficient, rapid, simple and green preparation technique.
分类号: O65
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