Identification of Increased Grain Length 1 (IGL1), a novel gene encoded by a major QTL for modulating grain length in rice

文献类型： 外文期刊

第一作者： Niu, Jiayu

作者： Niu, Jiayu;Wang, Fei;Yang, Chengcheng;Ye, Qiwen;Huang, Jingxian;La, Yumei;Wang, Qianqian;Hu, Tiange;Sang, Liran;Chen, Huhui;La, Honggui;Dai, Jie;Zhang, Peijiang;Zou, Yu;Zhai, Zhaoyu;Jin, Jian;Abdulmajid, Dina;Guo, Jingjing

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 1 期

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收录情况： SCI

摘要： Grain length is a prominent determinant for grain weight and appearance quality of rice. In this study, we conducted quantitative trait locus mapping to determine a genomic interval responsible for a long-grain phenotype observed in a japonica cultivar HD385. This led to the identification of a novel QTL for grain length on chromosome 3, named qIGL1 (for Increased Grain Length 1); the HD385 (Handao 385)-derived allele showed enhancement effects on grain length, and such an allele as well as NIP (Nipponbare)-derived allele was designated qigl1(HD385) and qIGL1(NIP), respectively. Genetic analysis revealed that the qigl1(HD385) allele displayed semidominant effects on grain length. Fine mapping further narrowed down the qIGL1 to an similar to 70.8-kb region containing 9 open reading frames (ORFs). A comprehensive analysis indicated that LOC_Os03g30530, which corresponded to ORF6 and carried base substitutions and deletions in HD385 relative to NIP, thereby causing changes or losses of amino-acid residues, was the true gene for qIGL1. Comparison of grain traits between a pair of near-isogenic lines (NILs), termed NIL-igl1(HD385) and NIL-IGL1(NIP), discovered that introduction of the igl1(HD385) into the NIP background significantly resulted in the elevations of grain length and 1000-grain weight. Closer inspection of grain surfaces revealed that the cell length and width in the longitudinal direction were significantly longer and greater, respectively, in NIL-igl1(HD385) line compared with in NIL-IGL1(NIP) line. Hence, our studies identified a new semidominant natural allele contributing to the increase of grain length and further shed light on the regulatory mechanisms of grain length.

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