"Blue-red-purple" multicolored lateral flow immunoassay for simultaneous detection of GM crops utilizing RPA and CRISPR/Cas12a

文献类型： 外文期刊

第一作者： Wang, Jinbin

作者： Wang, Jinbin;Luo, Jiawei;Liu, Hua;Xu, Danhong;Li, You;Zeng, Haijuan;Wang, Jinbin;Liu, Hua;Zeng, Haijuan;Luo, Jiawei;Liu, Xiaofeng;Zeng, Haijuan

作者机构：

关键词： Multicolored lateral flow immunoassay; Simultaneous detection; RPA; CRISPR/Cas12a; GM crops

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）

ISSN： 0039-9140

年卷期： 2025 年 282 卷

页码：

收录情况： SCI

摘要： Advanced multiplexed testing techniques should be designed and developed to ensure an accurate and reliable evaluation for unknown samples. In this study, an efficient platform coupled with the "Blue-Red-Purple" strategy based on recombinant polymerase amplification (RPA), CRISPR/Cas12a and lateral flow strip was established, which could realize the dual-target detection of CP4-EPSPS and Cry1Ab/Ac in genetically modified crops. The lateral flow immunoassay was developed using different colored microspheres to label the antibodies to realize the visualization of results and avoid cross-reactions. The proposed method exhibits high specificity, sensitivity and stability. The visual detection limits of standard plasmids and real samples reached 10 copies/mu L and 0.5 %, respectively, which could be stored at 4 degrees C for 12 months with high detection ability. Moreover, the entire detection process could be completed within 50 min without any complex instruments or professional operators. These findings indicated that a sensitive, specific, rapid and accurate method was established for on-site detection of GM crops.

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