First Report on Vibrio harveyi Infection in Black Rockfish (Sebastes schlegeli): Skin Ulcers, Histopathology, and Immune Response

文献类型： 外文期刊

第一作者： Gu, Yindong

作者： Gu, Yindong;Qin, Lei;Gu, Yindong;Yu, Yongxiang;Wang, Chunyuan;Wang, Yingeng;Zhang, Zhiqi;Rong, Xiaojun;Liao, Meijie;Li, Bin;Zhang, Zheng;Yu, Yongxiang;Wang, Yingeng;Rong, Xiaojun;Li, Bin;Zhang, Zheng

作者机构：

关键词： histopathology; mariculture; Sebastes schlegeli; skin ulcers; Vibrio harveyi

期刊名称：AQUACULTURE RESEARCH （ 影响因子：1.9； 五年影响因子：2.2 ）

ISSN： 1355-557X

年卷期： 2025 年 2025 卷 1 期

页码：

收录情况： SCI

摘要： In August 2023, a severe skin ulcer outbreak occurred in net-caged black rockfish (Sebastes schlegeli) of Nanhuangcheng Island, Bohai Sea. The causative bacterium, Vibrio harveyi, was identified through 16S rDNA and gyrB sequencing. Artificial infection experiments confirmed its high pathogenicity with a 7-day LD50 of 5.41 x 106 CFU/mL. Histopathological analysis revealed hepatocyte vacuolization, erythrocyte aggregation in the spleen leading to splenic congestion and reducing the contrast between red and white pulp regions, glomerular atrophy, renal damage, intestinal epithelial necrosis, and muscle fiber disintegration. Immunoenzymatic assays showed elevated alkaline phosphatase (AKP), acid phosphatase (ACP), and lysozyme (LZM) activity. Additionally, qRT-PCR analysis revealed downregulation of IL-12B and upregulation of TNF-alpha, CASP10, and RAFEA. Antibiotic susceptibility testing showed sensitivity to minocycline and doxycycline, but resistance to erythromycin and azithromycin. This study provides novel etiological and histopathological insights into V. harveyi infection in black rockfish, highlighting the bacterium's impact on host tissues and immune response.

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