Diel dynamics of multi-omics in elkhorn fern provide new insights into weak CAM photosynthesis

文献类型： 外文期刊

第一作者： Li, Cheng

作者： Li, Cheng;Han, Xiaoxu;He, Weijun;Nie, Bao;Bai, Wenhui;Zhang, Xiaopeng;He, Jingjing;Zhao, Cheng;Wang, Li;Huang, Wenjie;Zhang, Wenyang;Chen, Xufeng;Yan, Shijuan;Tschaplinski, Timothy J.;Yang, Xiaohan;Tschaplinski, Timothy J.;Yang, Xiaohan;Fernie, Alisdair R.;Zhao, Guohua;Zhang, Taijie;Wang, Li

作者机构：

关键词： Platycerium bifurcatum; crassulacean acid metabolism; multi-omics; transcription factor; PPCK; convergent evolution

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2023 年 4 卷 5 期

页码：

收录情况： SCI

摘要： Crassulacean acid metabolism (CAM) has high water-use efficiency (WUE) and is widely recognized to have evolved from C3 photosynthesis. Different plant lineages have convergently evolved CAM, but the molec-ular mechanism that underlies C3-to-CAM evolution remains to be clarified. Platycerium bifurcatum (elk -horn fern) provides an opportunity to study the molecular changes underlying the transition from C3 to CAM photosynthesis because both modes of photosynthesis occur in this species, with sporotrophophyll leaves (SLs) and cover leaves (CLs) performing C3 and weak CAM photosynthesis, respectively. Here, we report that the physiological and biochemical attributes of CAM in weak CAM-performing CLs differed from those in strong CAM species. We investigated the diel dynamics of the metabolome, proteome, and transcriptome in these dimorphic leaves within the same genetic background and under identical environ-mental conditions. We found that multi-omic diel dynamics in P. bifurcatum exhibit both tissue and diel ef-fects. Our analysis revealed temporal rewiring of biochemistry relevant to the energy-producing pathway (TCA cycle), CAM pathway, and stomatal movement in CLs compared with SLs. We also confirmed that PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE (PPCK) exhibits convergence in gene expression among highly divergent CAM lineages. Gene regulatory network analysis identified candidate transcription factors regulating the CAM pathway and stomatal movement. Taken together, our results provide new in-sights into weak CAM photosynthesis and new avenues for CAM bioengineering.

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