Novel Allelic Mutations in Dw3 Gene That Affect the Height of Sorghum Plants

文献类型： 外文期刊

第一作者： Wang, Ping

作者： Wang, Ping;Liang, Bingbing;Li, Zhengjun;Wang, Chunyu;Zhang, Lixia;Lu, Xiaochun

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关键词： BSA-seq; Dw3 gene; novel mutations; sorghum

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 22 期

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收录情况： SCI

摘要： Breeding for dwarfing traits in sorghum is crucial. However, only three genes (Dw1-Dw3) that control plant height have been mapped. In this study, 634 sorghum cultivars were collected to investigate plant height and genotypes. Four were genotyped Dw1DW2Dw3 (wild type) but with different plant heights, and they were selected to construct two populations and map new dwarf genes. Bulked segregant analysis with whole-genome resequencing of the two populations identified the candidate gene in one same genomic region-on chromosome 7. Then, it was narrowed down to a region containing nine genes. Amino acid and DNA sequence analysis of the parent and offspring plants revealed that two novel allelic mutations in the Dw3 gene play a role in reducing the plant height-8R262 or 8R417, including 1 bp substitution and 2 bp deletions. Furthermore, we sequenced 19 cultivars that primarily exhibited a "one-dwarf" hybrid or wild-type and presumed another allelic mutation via the amino acid alignment of 8R019, 8R100, and 8R402, which was another one-base substitution. These results indicate that multiple types of allelic mutations in the Dw3 gene should be considered when identified or applied.

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