Sensitive and rapid detection of Karenia mikimotoi (Dinophyceae) by loop-mediated isothermal amplification

文献类型： 外文期刊

第一作者： Zhang, Fengying

作者： Zhang, Fengying;Ma, Lingbo;Xu, Zhaoli;Zheng, Junbin;Shi, Yanhong;Lu, Yanan;Miao, Yuping

作者机构：

关键词： identification;karenia mikimotoi;loop-mediated isothermal amplification (LAMP);Ribosomal DNA internal transcribed spacer (ITS)

期刊名称：HARMFUL ALGAE （ 影响因子：4.273； 五年影响因子：5.824 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A loop-mediated isothermal amplification (LAMP) assay was developed to detect the genomic DNA of Karenia mikimotoi using a set of four specific primers based on a ribosomal DNA internal transcribed spacer (ITS). The sensitivity of this LAMP assay was 100-fold higher than regular PCR, and its specificity was validated using other algae as a comparison. Two visual detection approaches were feasible to interpret the positive or negative results. This technology may have the potential to aid in forecasting red-tides on the scene because of its high sensitivity, specificity and rapid detection.

分类号： Q17

  • 相关文献

[1]GROWTH INTERACTIONS BETWEEN THE BLOOM-FORMING DINOFLAGELLATES Prorocentrum donghaiense AND Karenia mikimotoi UNDER DIFFERENT TEMPERATURE. Shen, Anglu,Liu, Guangpeng,Li, Daoji,Shen, Anglu,Yuan, Xingwei. 2014

[2]Loop-mediated isothermal amplification assays for screening of bacterial integrons. Yu, Guangchao,Yu, Guangchao,Li, Bing,Cui, Hemiao,Chen, Siyi,Miao, Jian,Deng, Yang,Chen, Lei,Lin, Chii-wann,Bian, Huawei,Chen, Dingqiang. 2014

[3]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[4]Rapid Detection of Listeria monocytogenes in Raw Milk with Loop-Mediated Isothermal Amplification and Chemosensor. Wang, Deguo,Zhang, Gaiping,Deng, Ruiguang,Zhi, Aimin,Guo, Junqing,Zhao, Dong,Wang, Deguo,Xu, Zhihong,Lu, Chengping. 2011

[5]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[6]Loop-mediated isothermal amplification for detection of porcine circovirus type 2. Han, Si,Shi, Jianli,Wu, Jiaqiang,Yuan, Xiaoyuan,Cong, Xiaoyan,Xu, Shaojian,Wu, Xiaoyan,Li, Jun,Wang, Jinbao,Zhou, Shun,Han, Si,Wu, Xiaoyan,Yuan, Xiaoyuan,Li, Jun,Wang, Jinbao. 2011

[7]Application of an improved loop-mediated isothermal amplification detection of Vibrio parahaemolyticus from various seafood samples. Li, Chunhou,Xu, Zhenbo,Wang, Li,Li, Yue,Zhong, Qingping,Zhao, Xihong,Xu, Zhenbo,Chu, Jin,Li, Yanyan. 2011

[8]Detection of LAMP Products Using CNT Array Electrode. Zhang, Xuzhi,Li, Qiufen,Chen, Bijiuan,Ma, Shaosai,Cui, Yi. 2011

[9]Development of a loop-mediated isothermal amplification assay for rapid detection of iridovirus in the Chinese giant salamander. Meng, Yan,Zhang, Hui,Liang, Hongwei,Zeng, Lingbing,Xiao, Hanbing,Meng, Yan,Xie, Congxin. 2013

[10]Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 DNA. Ren, Weicheng,Cai, Yuyong,Wang, Chongming,Renault, Tristan,Cai, Yuyong. 2010

[11]Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Xiao, Shao-bo. 2012

[12]Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification. Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo,Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo. 2012

[13]Rapid and Sensitive Detection of sFAT-1 Transgenic Pigs by Visual Loop-Mediated Isothermal Amplification. Tao, Chenyu,Yang, Yalan,Li, Xunbi,Li, Kui,Zhou, Rong,Tao, Chenyu,Yang, Yalan,Li, Xunbi,Li, Kui,Zhou, Rong,Tao, Chenyu,Zhou, Rong,Zheng, Xinmin,Ren, Hongyan.

[14]Reverse transcription loop-mediated isothermal amplification of DNA for detection of Barley yellow dwarf viruses in China. Zhao, Kun,Liu, Yan,Wang, Xifeng. 2010

[15]Comparison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Lin, Zhibing,Zhang, Yanlei,Zhang, Houshuang,Zhou, Yongzhi,Cao, Jie,Zhou, Jinlin.

[16]Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR. Wang, Yanhua,Wang, Guangxiang,Wang, Meng,Zhang, Delin,Yin, Hong.

[17]Comparison of a loop-mediated isothermal amplification for orf virus with quantitative real-time PCR. Wang, Guangxiang,Wang, Yanhua,Liu, Xiangtao,Tian, Hong,Shang, Youjun,Wang, Guangxiang,Shang, Youjun,Wang, Yanhua,Liu, Xiangtao. 2013

[18]Detection of mink enteritis virus by loop-mediated isothermal amplification (LAMP). Wang, Jianke,Cheng, Shipeng,Yi, Li,Cheng, Yuening,Yang, Shen,Xu, Hongli,Li, Zhenguang,Shi, Xinchuan,Wu, Hua,Yan, Xijun. 2013

[19]Evaluation of loop-mediated isothermal amplification (LAMP) assays based on 5S rDNA-IGS2 regions for detecting Meloidogyne enterolobii. Niu, J. H.,Jian, H.,Guo, Q. X.,Chen, C. L.,Wang, X. Y.,Liu, Q.,Niu, J. H.,Niu, J. H.,Guo, Y. D.. 2012

[20]Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. Liu, Da-Fei,Chai, Hong-Liang,Yin, Xiu-Chen,Hua, Yu-Ping,Liu, Da-Fei,Liu, Chun-Guo,Tian, Jin,Zhang, Xiao-Zhan,Yang, Tian-Kuo,Zhang, Hong-Ying,Liu, Ming,Qu, Lian-Dong,Jiang, Yi-Tong.

作者其他论文 更多>>

微信小程序