Unveiling side-chain architectures of corn husk hemicellulose to drive enzyme cocktail design for degradation of biomass

文献类型： 外文期刊

第一作者： Guo, Yingjie

作者： Guo, Yingjie;Zhang, Zheng;Li, Xuezhi;Zhao, Jian;Guo, Yingjie

作者机构：

关键词： Corn husk hemicellulose; Structural characterization; One-step enzymatic hydrolysis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 310 卷

页码：

收录情况： SCI

摘要： By analyzing residual hemicellulose after enzymatic hydrolysis of corn husks, we identified previously unreported side-chain architectures in glucuronoarabinoxylan and xyloglucan of corn husk hemicellulose, including alpha-galactosyl-mannose and alpha-xylosyl-arabinose linkages, which shield glycosidic bonds from enzymatic cleavage. These discoveries unveil novel structural complexes in hemicellulose and broaden the understanding of hemicellulose recalcitrance beyond existing literature. Further, we designed a tailored enzyme cocktail by heterologously expressing three targeted hemicellulases (GH51 alpha-L-arabinofuranosidase, GH27 alpha-D-galactosidase, and GH31 alpha-D-xylosidase) based on hemicellulose structural information, and synergistic integration of these enzymes with Penicillium oxalicum cellulase via response surface optimization. This enzyme system achieved unprecedented yields of 81.82 % glucose and 54.89 % arabinose through one-step hydrolysis without chemical pretreatment, surpassing previous benchmarks by over 30 %. This study advances the understanding of hemicellulose's intricate structural complexity and enhances insights into multi-enzyme synergies during biomass degradation, offering a pivotal framework for developing pretreatment-free, eco-friendly enzymatic hydrolysis processes and enabling high-value utilization of corn husks.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序