Developing an Optimized Protocol for Regeneration and Transformation in Pepper

文献类型： 外文期刊

第一作者： Shams, Shamsullah

作者： Shams, Shamsullah;Naeem, Beenish;Ma, Lingling;Zhang, Zhenghai;Cao, Yacong;Yu, Hailong;Feng, Xigang;Wang, Lihao;Ma, Lingling;Li, Rongxuan;Wu, Huamao;Ma, Lingling;Qiu, Yinhui

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关键词： Capsicum annuum; in vitro culture; Agrobacterium tumefaciens; explants

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2024 年 15 卷 8 期

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收录情况： SCI

摘要： Capsicum annuum L. is extensively cultivated in subtropical and temperate regions globally, respectively, when grown in a medium with 8 holding significant economic importance. Despite the availability of genome sequences and editing tools, gene editing in peppers is limited by the lack of a stable regeneration and transformation method. This study assessed regeneration and transformation protocols in seven chili pepper varieties, including CM334, Zunla-1, Zhongjiao6 (ZJ6), 0818, 0819, 297, and 348, in order to enhance genetic improvement efforts. Several explants, media compositions, and hormonal combinations were systematically evaluated to optimize the in vitro regeneration process across different chili pepper varieties. The optimal concentrations for shoot formation, shoot elongation, and rooting in regeneration experiments were determined as 5 mg/L of 6-Benzylaminopurine (BAP) with 5 mg/L of silver nitrate (AgNO3), 0.5 mg/L of Gibberellic acid (GA(3)), and 1 mg/L of Indole-3-butyric acid (IBA), respectively. The highest regeneration rate of 41% was observed from CM334 cotyledon explants. Transformation optimization established 300 mg/L of cefotaxime for bacterial control, with a 72-h co-cultivation period at OD600 = 0.1. This study optimizes the protocols for chili pepper regeneration and transformation, thereby contributing to genetic improvement efforts.

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