Molecular and functional characterization of a novel stefin analogue in large yellow croaker (Pseudosciaena crocea)

文献类型： 外文期刊

第一作者： Li, Shuying

作者： Li, Shuying;Yang, Zhijun;Ao, Jingqun;Chen, Xinhua;Li, Shuying;Li, Shuying

作者机构：

关键词： Large yellow croaker Pseudosciaena crocea;Stefin;Subcellular;localization;Protease-inhibitory activity;Expression modulation

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this paper, we report the molecular cloning of a novel stefin analogue from the spleen of large yellow croaker Pseudosciaena crocea (Lycstefin). The open reading frame (ORF) of 297 nucleotides (nt) of Lycstefin encodes a protein of 99 amino acids (aa) with a putative molecular weight of 11 kDa, in which no signal peptide and potential N-glycoslation site are predicted. The deduced Lycstefin possesses the structural features of the mammalian stefins, including two conserved motifs known to interact with the active sites of family C1 cysteine peptidases: one glycine in the N-terminal region (G(6)) and Gln-Xaa-Val-Xaa-Gly motif (Q(48)LVAG(52)). It shares 32-47.5% aa sequence identity to the sequences found in mammals and other fish species and is rich in cysteine residues (seven cysteines). Genomic analysis revealed that Lyccys gene, 757 nt long, consisted of three exons and two introns. The Lycstefin gene was constitutively expressed in various tissues examined although at different levels. Upon stimulation with poly(I:C) or inactivated trivalent bacterial vaccine, Lycstefin transcript was significantly tip-regulated in spleen and head kidney while down-regulated in blood. Immuno-electron microscopy showed that Lycstefin was mainly localized in the cytoplasm of spleen cells of large yellow croaker, and also in the nucleus. Recombinant Lycstefin protein fused with glutathione S-transferase (rLycstefin) was shown to have strong inhibitory activity against papain with a K-i of 1.3 x 10(-13) M. The in vivo experiments revealed that Lycstefin could not modulate the expression levels of large yellow croaker tumor necrosis factor-alpha 2 (TNF-alpha 2) and interleukin-10 in spleen and head kidney. To our knowledge, this is the first report on the molecular and functional identification of a stefin analogue in bony fish.

分类号： R392

  • 相关文献

[1]Molecular characterization and expression analysis of beta(2)-microglobulin in large yellow croaker Pseudosciaena crocea. Yu, Suhong,Chen, Xinhua,Ao, Jingqun,Yu, Suhong,Yu, Suhong. 2009

[2]Molecular characterization and expression analysis of MHC class II alpha and beta genes in large yellow croaker (Pseudosciaena crocea). Yu, Suhong,Ao, Jingqun,Chen, Xinhua,Yu, Suhong,Yu, Suhong. 2010

[3]Molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells. Gao, Quanxin,Zhang, Chenjie,Min, Minghua,Peng, Shiming,Shi, Zhaohong,Xiao, Yingping.

[4]Accumulation and subcellular localization of heat shock proteins in young grape leaves during cross-adaptation to temperature stresses. Zhang, Jun-Huan,Pan, Qiu-Hong,Zhan, Ji-Cheng,Huang, Wei-Dong,Zhang, Jun-Huan,Wang, Yu-Zhu,Wang, Li-Jun. 2008

[5]Localization, Expression Change in PRRSV Infection and Association Analysis of the Porcine TAP1 Gene. Sun, Nunu,Liu, Dewu,Meng, Fanming,Zhang, Xianwei,Wu, Zhenfang,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Chen, Hongbo,Chen, Huiyong. 2012

[6]BP Neural Network Based Localization for a Front-Wheel Drive and Differential Steering Mobile Robot. Jia, Shiwei,Qiu, Quan,Li, You,Cong, Yue,Jia, Shiwei,Qiu, Quan,Li, You,Cong, Yue,Jia, Shiwei,Li, Junmin. 2015

[7]Characterization and mapping of the bovine FBP1 gene. Guo, H.,Liu, W-S.,Takasuga, A.,Eyer, K.,Landrito, E.,Xu, S-Z.,Gao, X.,Ren, H-Y.. 2007

[8]Protective efficacy of recombinant Cryptosporidium parvum CpPRP1 sushi domain against C-tyzzeri infection in mice. Huang, Y.,Cao, W.,Shi, K.,Mi, R.,Lu, K.,Han, X.,Chen, Z.,Huang, Y.,Mi, R.,Lu, K.,Han, X.,Chen, Z..

[9]The asparagine-rich protein NRP interacts with the Verticillium effector PevD1 and regulates the subcellular localization of cryptochrome 2. Zhou, Ruimin,Zhu, Tong,Xu, Mengyuan,Liu, Yanli,Han, Dandan,Liu, Xinqi,Han, Lei,Liu, Mengjie,Qiu, Dewen,Gong, Qingqiu.

[10]A simple, rapid, and reliable protocol to localize hydrogen peroxide in large plant organs by DAB-mediated tissue printing. Liu, Yong-Hua,Offler, Christina E.,Ruan, Yong-Ling,Liu, Yong-Hua,Offler, Christina E.,Ruan, Yong-Ling,Liu, Yong-Hua. 2014

[11]Sequence Analysis and Molecular Characterization of Wnt4 Gene in Metacestodes of Taenia solium. Hou, Junling,Luo, Xuenong,Wang, Shuai,Yin, Cai,Zhang, Shaohua,Zhu, Xueliang,Dou, Yongxi,Cai, Xuepeng.

[12]MOLECULAR CHARACTERIZATION OF ARACHIS HYPOGAEA NAC 2 (AHNAC2) REVEALS IT AS A NAC-LIKE PROTEIN IN PEANUT. Liu, X.,Zhang, B. Y.,Hong, L.,Su, L. C.,Li, L.,Liang, X. Q.,Li, X. Y.. 2010

[13]A broadly tuned odorant receptor in neurons of trichoid sensilla in locust, Locusta migratoria. You, Yinwei,Lv, Mingyue,Zhang, Long,You, Yinwei,Smith, Dean P.,Smith, Dean P..

[14]A Localization Algorithm for Sparse-Anchored WSN in Agriculture. Zhao, Chunjiang,Wang, Shufeng,Wang, Kaiyi,Liu, Zhongqiang,Yang, Feng,Zhang, Xiandi. 2011

[15]Relative abundance of G protein-coupled receptor 30 and localization in testis and epididymis of sheep at different developmental stages. Lu, Peiyao,Wang, Fuchuan,Song, Xianyi,Liu, Yang,Zhang, Kai,Cao, Ningxian.

[16]Neuropeptide Y content in the hypothalamic paraventricular nucleus responds to fasting and refeeding in broiler chickens. Zhou, WD,Murakami, M,Hasegawa, S,Yoshizawa, F,Sugahara, K.

[17]Expression characterization, polymorphism and chromosomal location of the porcine calsarcin-3 gene. Wang, Heng,Yang, Shulin,Tang, Zhonglin,Mu, Yulian,Cui, Wentao,Li, Kui. 2007

[18]Characterization of -catenin 1 during the gonad development in the common carp (Cyprinus carpio). Wang, Lan-mei,Zhu, Wen-bin,Fu, Jian-jun,Dong, Zai-jie,Song, Fei-biao,Dong, Juan-juan,Dong, Zai-jie.

[19]Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence. Li, RJ,Hua, W,Lu, YT.

[20]Manipulating volatile emission in tobacco leaves by expressing Aspergillus niger beta-glucosidase in different subcellular compartments. Wei, S,Marton, I,Dekel, M,Shalitin, D,Lewinsohn, E,Bravdo, BA,Shoseyov, O.

作者其他论文 更多>>

微信小程序