microRNA-430b targets scavenger receptor class B member 1 (scarb1) and inhibits coloration and carotenoid synthesis in koi carp (Cyprinus carpio L.)

文献类型： 外文期刊

第一作者： Tian, Xue

作者： Tian, Xue;Nian-nian Peng;Ma, Xiao;Li-min Wu;Shi, Xi;Hui-fen Liu;Xiao-lin Meng;Xue-jun Li;Tian, Xue;Ma, Xiao;Li-min Wu;Shi, Xi;Hui-fen Liu;Xiao-lin Meng;Xue-jun Li;Hong-mei Song;Qi-sheng Wu

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关键词： miR-430; scarb1; Carotenoid metabolism; Skin color; Koi carp

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2022 年 546 卷

页码：

收录情况： SCI

摘要： In teleost fishes, miR-430 has several paralogs and is widely required for mesoderm formation and gonad development. However, the functions of miR-430 in skin pigmentation have not been completely elucidated. To understand the functions of miR-430 in color formation, we analyzed the conserved characteristics of miR-430 family in teleost, detected miR-430 family spatial and temporal expression patterns, verified the target differences between miR-430a and miR-430b, identified the phenotypic changes by overexpressing miR-430b in vivo and assessed the roles of miR-430b in coloration by analyzing the genes associated with pteridine and carotenoid pigment metabolism. The results showed that miR-430a and miR-430b were highly conserved in teleosts and that miR-430b was intensively expressed in skin, fins and scales comparing with miR-430a. Target verification analysis showed that miR-430b displayed significant repressive effects on scarb1 compared with miR-430a. In addition, overexpression of miR-430b led to diminished red intensity by decreasing skin carotenoid content and xanthophores/erythrophores density in scales. Accompanying the color dilution, the mRNA and protein levels of scarb1 were markedly inhibited by increased miR-430b, and several genes correlated with carotenoid metabolism also underwent corresponding modification. However, the contents of pteridine pigments and related genes experienced only limited changes. These findings suggest that miR-430b can improve skin pigmentation by blocking the expression of scarb1, resulting in carotenoid metabolism dysregulation and chromatophores density reduction.

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