Lipase Diversity in Glacier Soil Based on Analysis of Metagenomic DNA Fragments and Cell Culture

文献类型： 外文期刊

第一作者： Zhang Yuhong

作者： Zhang Yuhong;Shi, Pengjun;Meng, Kun;Bai, Yingguo;Wang, Guozeng;Yao, Bin;Zhang Yuhong;Liu, Wanli;Zhan, Zhichun

作者机构：

关键词： Lipase;gene diversity;metagenomic library;sequence-based screening;glacier soil

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Lipase diversity in glacier soil was assessed by culture-independent metagenomic DNA fragment screening and confirmed by cell culture experiments. A set of degenerate PCR primers specific for lipases of the hormone-sensitive lipase family was designed based on conserved motifs and used to directly PCR amplify metagenomic DNA from glacier soil. These products were used to construct a lipase fragment clone library. Among the 300 clones sequenced for the analysis, 201 clones encoding partial lipases shared 51-82% identity to known lipases in GenBank. Based on a phylogenetic analysis, five divergent clusters were established, one of which may represent a previously unidentified lipase subfamily. In the culture study, It lipase-producing bacteria were selectively isolated and characterized by 16S rDNA sequences. Using the above-mentioned degenerate primers, seven lipase gene fragments were cloned, but not all of them could be accounted for by the clones in the library. Two full-length lipase genes obtained by TAIL-PCR were expressed in Pichia pastoris and characterized. Both were authentic lipases with optimum temperatures of <= 40 degrees C. Our study indicates the abundant lipase diversity in glacier soil as well as the feasibility of sequence-based screening in discovering new lipase genes from complex environmental samples.

分类号： Q93

  • 相关文献

[1]Molecular Cloning and Characterization of a Novel Cold-Adapted Family VIII Esterase from a Biogas Slurry Metagenomic Library. Cheng, Xiaojie,Wang, Xuming,Qiu, Tianlei,Gao, Junlian,Yuan, Mei,Sun, Jianguang.

[2]A novel feruloyl esterase from a soil metagenomic library with tannase activity. Yao, Jian,Chen, Qing Long,Shen, Ai Xi,Cao, Wen,Yao, Jian,Liu, Yu Huan.

[3]Isolation of a novel alkaline-stable lipase from a metagenomic library and its specific application for milkfat flavor production. Peng, Qing,Wang, Xu,Shang, Meng,Huang, Jinjin,Guan, Guohua,Li, Ying,Peng, Qing,Wang, Xu,Shang, Meng,Huang, Jinjin,Guan, Guohua,Li, Ying,Peng, Qing,Shi, Bo. 2014

[4]A novel efficient beta-glucanase from a paddy soil microbial metagenome with versatile activities. Zhou, Yu,Wang, Xu,Xie, Zhongwen,Zhang, Zhengzhu,Wei, Chaoling,Wei, Wei,Xu, Jimin,Wang, Wei,Jiang, Hongchen,Wang, Qi. 2016

[5]Isolation and characterization of a novel cyanophycin synthetase from a deep-sea sediment metagenomic library. Du, Jikun,Lu, Yongjun,Zhou, Shining,Li, Li,Ding, Xian,Hu, Haiyan,Zhou, Shining. 2013

[6]Metagenomic cloning and characterization of Na+/H+ antiporter genes taken from sediments in Chaerhan Salt Lake in China. Wang, Xiaoyu,Chen, Sanfeng,Xu, Feng.

[7]Gene flow among different teosinte taxa and into the domesticated maize gene pool. Warburton, Marilyn L.,Wilkes, Garrison,Taba, S.,Dreisigacker, Susanne,Bedoya, Claudia,Prasanna, B. M.,Charcosset, Alain,Mir, Celine,Dumas, Fabrice,Madur, Delphine,Xie, C. X.,Hearne, Sarah,Franco, Jorge.

[8]Genetic characteristics of hybrid populations derived by crossing Chinese and Indian pearl oysters, Pinctada fucata, based on AFLP markers. Yu, Da Hui,Huang, Gui-ju,Guo, Yi-hui,Wang, Xiao-yu,Wang, Ai-min. 2009

[9]Molecular detection and diversity of xylanase genes in alpine tundra soil. Wang, Guozeng,Wang, Yaru,Yang, Peilong,Luo, Huiying,Huang, Huoqing,Shi, Pengjun,Meng, Kun,Yao, Bin.

[10]Expressed sequence tag-simple sequence repeat-based molecular variance in two Salicornia (Amaranthaceae) populations. Xu, Z. L.,Ali, Z.,Yi, J. X.,He, X. L.,Zhang, D. Y.,Yu, G. H.,Ma, H. X.,Ali, Z.,Khan, A. A.,Ali, Z.,Khan, A. A.,Khan, I. A.. 2011

[11]Host Specificity of Flagellins from Segmented Filamentous Bacteria Affects Their Patterns of Interaction with Mouse Ileal Mucosal Proteins. Chen, Huahai,Wang, Yanling,Xiang, Charlie,Chen, Huahai,Wang, Yanling,Xiang, Charlie,Chen, Huahai,Yin, Yeshi,Wang, Xin,Chen, Huahai,Yin, Yeshi,Wang, Xin. 2017

[12]Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53. Zhang, Zengyan,Xin, Zhiyong,Li, Tao,Hu, Yingkao,Duan, Xiayu.

[13]Genetic Diversity and Geographical Differentiation of Desmodium triflorum (L.) DC. in South China Revealed by AFLP Markers. Yue, Mao-feng,Zhou, Ren-chao,Huang, Ye-lin,Xin, Guo-rong,Shi, Su-hua,Yue, Mao-feng,Feng, Li.

[14]Evaluation of the genetic diversity and genome-wide linkage disequilibrium of Chinese maize inbred lines. Wang, Ming,Zhang, Xiaobo,Zheng, Yonglian,Zhao, Jiuran,Song, Wei. 2011

[15]Effects of DIMBOA on several enzymatic systems in Asian corn borer, Ostrinia furnacalis (Guenee). Yan, FM,Xu, CG,Li, SG,Lin, CS,Li, JH. 1995

[16]Isolation and biochemical characterization of two lipases from a metagenomic library of China Holstein cow rumen. Liu, Kailang,Wang, Jiaqi,Bu, Dengpan,Zhao, Shengguo,Yu, Ping,Li, Dan,McSweeney, Chris. 2009

[17]Progress of Lipase-Catalyzed Ester Synthesis in Ionic Liquid. Li Jing,Wang Jun,Zhang Leixia,Gu Shuangshuang,Wu Fuan,Guo Yuewei,Wu Fuan,Guo Yuewei. 2012

[18]A novel chemoenzymatic synthesis of propyl caffeate using lipase-catalyzed transesterification in ionic liquid. Pang, Na,Gu, Shuang-Shuang,Wang, Jun,Cui, Hong-Sheng,Wang, Fang-Qin,Liu, Xi,Zhao, Xing-Yu,Wu, Fu-An,Wu, Fu-An.

[19]Pretreating porcine sperm with lipase enhances developmental competence of embryos produced by intracytoplasmic sperm injection. Wei, Yinghui,Fan, Junhua,Li, Lin,Liu, Zhiguo,Li, Kui.

[20]A novel proteolysis-resistant lipase from keratinolytic Streptomyces fradiae var. k11. Zhang, Yuhong,Meng, Kun,Wang, Yaru,Luo, Huiying,Yang, Peilong,Shi, Pengjun,Yao, Bin,Wu, Ningfeng,Fan, Yunhu,Li, Jiang.

作者其他论文 更多>>

微信小程序