Protective effect of glutathione against oxidative injury in intestinal epithelial cells of piglet in vitro

文献类型： 外文期刊

第一作者： Li, Ying-Qiu

作者： Li, Ying-Qiu;Chen, Qun;Zhang, Shu-Ming;Liu, Hai-Yan

作者机构：

关键词： Antioxidant capacity;DNA injury;malondialdehyde;oxidative stress

期刊名称：ANIMAL BIOLOGY （ 影响因子：1.475； 五年影响因子：1.375 ）

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收录情况： SCI

摘要： Oxidative stress of intestinal epithelium is involved in inflammatory bowel disease. To investigate protective effects of glutathione (GSH) on xanthine/xanthine oxidase (X/XO)-induced oxidative injury in intestinal epithelial cells (IECs). We employed in vitro cell culture supplemented with X/XO. IECS were cultured for 72 h, and then divided into seven groups with various concentrations of X/XO and GSH supplementation in the medium. Agarose get electrophoresis lanes indicated that X/XO induced DNA injury by the high concentration of XO (40, 70 U/L)-treated groups. The X/XO supplementation significantly increased the production of malondialdehyde (MDA) in a dose-dependent manner. There was a slight increase in total radical-trapping antioxidant potential (TRAP) value by the low concentration of XO (10U/L) alone-treated group (P > 0.05) while supplementation of a high concentration of XO (40, 70 U/L) significantly decreased TRAP value compared with XO (10 U/L) and the control group (P < 0.05). Addition of GSH decreased the production of MDA and DNA fragmentations (P < 0.05), but enhanced TRAP value (P < 0.05). These results suggest that IECs of piglet have the ability of enduring mild oxidative stress induced by a low concentration of XO. Although high concentrations of XO resulted in oxidation injury and lipid peroxidation in the IECs, additions of GSH to the medium showed significant protection against the X/XO-induced oxidative stress.

分类号： Q95

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