Molecular characterization of an ethephon-induced Hsp70 involved in high and low-temperature responses in Hevea brasiliensis

文献类型： 外文期刊

第一作者： Zhang, Zhi-Li

作者： Zhang, Zhi-Li;Zhu, Jia-Hong;Zhang, Quan-Q I.;Cai, Yuan-Bao;Zhang, Zhi-Li

作者机构：

关键词： Hevea brasiliens;Heat shock protein 70;Ethephon;Expression;Low;temperature

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Hsp70s have been shown to play important roles in helping cells to cope with adverse environments, especially in response to temperature. In this study a novel ethephon-induced Hsp gene, designated as HbHsp70, was isolated from Hevea brasiliensis. The HbHsp70 cDNA contained a 1965 bp open reading frame encoding 655 amino acids. The deduced HbHsp70 protein showed high identities to Hsp70s from other plants. Expression studies revealed more significant accumulation of HbHsp70 transcripts in leaves and stems than in roots, barks and latex. The transcription of HbHsp70 was induced by ethephon, heat treatment and low temperature stress, whereas jasmonic acid had little effects. Recombinant HbHsp70 was expressed in Escherichia coli and purified by Ni-NTA affinity chromatography. Measuring the light scattering of luciferase (Luc) revealed that HbHsp70 prevents the aggregation of luc during high-temperature stress. In vitro experiments showed that HbHsp70 had protective functions not only against heat stress but also against chilling stress. All these data suggest that HbHsp70 may play roles in responses to heat shock and low temperature in H. brasiliensis.

分类号： Q945

  • 相关文献

[1]Molecular Characterization of HbCDPK1, an Ethephon-Induced Calcium-Dependent Protein Kinase Gene of Hevea brasiliensis. Zhu, Jia-Hong,Chen, Xin,Chang, Wen-Jun,Zhang, Zhi-Li,Zhang, Zhi-Li,Zhu, Jia-Hong,Chen, Xin,Chang, Wen-Jun,Tian, Wei-Min.

[2]Influence of ethylene on adventitious root formation in mung bean hypocotyl cuttings. Pan, RC,Wang, JX,Tian, XS.

[3]De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction. Liu, Chuan-He,Fan, Chao,Liu, Chuan-He,Fan, Chao. 2016

[4]Involvement of Pheophytinase in Ethylene-Mediated Chlorophyll Degradation in the Peel of Harvested 'Yali' Pear. Cheng, Yudou,Guan, Junfeng. 2014

[5]Expression profiling of HbWRKY1, an ethephon-induced WRKY gene in latex from Hevea brasiliensis in responding to wounding and drought. Zhang, Quanqi,Zhu, Jiahong,Ni, Yanmei,Cai, Yuanbao,Zhang, Zhili,Zhang, Quanqi,Ni, Yanmei,Zhang, Zhili. 2012

[6]Molecular characterization and expression analysis of two farnesyl pyrophosphate synthase genes involved in rubber biosynthesis in Hevea brasiliensis. Wu, Chuntai,Sun, Lina,Li, Yu,Zeng, Rizhong,Wu, Chuntai,Sun, Lina,Li, Yu,Zeng, Rizhong. 2017

[7]Molecular characterization of HbEREBP2, a jasmonate-responsive transcription factor from Hevea brasiliensis Muell. Arg.. Chen, Yue-Yi,Wang, Li-Feng,Yang, Shu-Guang,Tian, Wei-Min,Chen, Yue-Yi. 2011

[8]HbMT2, an ethephon-induced metallothionein gene from Hevea brasiliensis responds to H2O2 stress. Zhu, Jiahong,Zhang, Quanqi,Wu, Rui,Zhang, Zhili,Zhang, Zhili. 2010

[9]Abscisic acid and ethephon regulation of cellulase in the endosperm cap and radicle during lettuce seed germination. Chen, Bingxian,Ma, Jun,Xu, Zhenjiang,Wang, Xiaofeng,Chen, Bingxian. 2016

[10]Molecular cloning and expression analysis of the mevalonate diphosphate decarboxylase gene from the latex of Hevea brasiliensis. Wu, Chuntai,Li, Yu,Nie, Zhiyi,Dai, Longjun,Kang, Guijuan,Zeng, Rizhong,Wu, Chuntai,Li, Yu,Nie, Zhiyi,Dai, Longjun,Kang, Guijuan,Zeng, Rizhong.

[11]Ethephon Increases Rubber Tree Latex Yield by Regulating Aquaporins and Alleviating the Tapping-Induced Local Increase in Latex Total Solid Content. An, Feng,Xie, Guishui,Zou, Zhi,An, Feng,Kong, Lingxue,Rookes, James,Cahill, David,Cai, Xiuqing.

[12]Expression of heat shock protein 70 in transport-stressed broiler pectoralis major muscle and its relationship with meat quality. Xing, T.,Han, M. Y.,Xu, X. L.,Zhou, G. H.,Wang, M. F.,Zhu, X. S.. 2017

[13]Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae). Chen, Hao-Liang,Zhangl, Hong-Yu,Chen, Hao-Liang,Chen, Hao-Liang,Throne, James E.,Chen, Hao-Liang,Zhu, Kun Yan. 2014

[14]Overexpression of heat shock protein 70 and its relationship to intestine under acute heat stress in broilers: 2. Intestinal oxidative stress. Gu, X. H.,Hao, Y.,Wang, X. L.. 2012

[15]Genomic, evolutionary and expression profile analysis of Hsp70 superfamily in A and D genome of cotton (Gossypium spp.) under the challenge of Verticillium dahliae. Xiao, Songhua,Yu, Jingzhong,Yu, Deyue,Xiao, Songhua,Xu, Jianwen,Zhao, Jun,Liu, Jianguang,Wu, Qiaojuan. 2017

[16]Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae. Qi, Zemin,Zou, Yuanchao,Yue, Xingjian,Xie, Biwen,Zhao, Daxian,Gong, Quan.

[17]SjHSP70, a recombinant Schistosoma japonicum heat shock protein 70, is immunostimulatory and induces protective immunity against cercarial challenge in mice. Duan, Ming Ming,Xu, Rui Min,Yuan, Chun Xiu,Li, Yun Yan,Liu, Qun,Cheng, Guo Feng,Lin, Jiao-Jiao,Feng, Xin Gang,Li, Yun Yan,Liu, Qun,Lin, Jiao-Jiao.

[18]AtFes1A is Essential for Highly Efficient Molecular Chaperone Function in Arabidopsis. Fu, Can,Liu, Xiaxia,Yang, Wenwen,Yu, Hongbin,Liu, Jian,Zhang, Jingxia.

[19]Eimeria tenella heat shock protein 70 enhances protection of recombinant microneme protein MIC2 subunit antigen vaccination against E. tenella challenge. Zhang, Lei,Ma, Liping,Liu, Renqiang,Zhang, Yunfei,Zhang, Shouping,Hu, Chunmei,Song, Meng,Wang, Ming,Cai, Jianping. 2012

[20]Overexpression of heat shock protein 70 and its relationship to intestine under acute heat stress in broilers: 1. Intestinal structure and digestive function. Hao, Y.,Gu, X. H.,Wang, X. L.. 2012

作者其他论文 更多>>

微信小程序