Immobilization of Chitosanase on Magnetic Nanoparticles: Preparation, Characterization and Properties

文献类型： 外文期刊

第一作者： Cheng Yimeng

作者： Cheng Yimeng;Liu Zhen;Mao Xiangzhao;Cheng Yimeng;Li Zhaoyue;Sun Huihui;Zhao Ling;Cao Rong;Liu Qi;Cao Rong;Mao Xiangzhao

作者机构：

关键词： chitosanase; immobilization; magnetic nanoparticles; chitooligosaccharides

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：1.179； 五年影响因子：1.225 ）

ISSN： 1672-5182

年卷期： 2022 年 21 卷 5 期

页码：

收录情况： SCI

摘要： Chitosanase could cleave beta-1,4-linkage of chitosan to produce chitooligosaccharides (COS) with diverse biological activities. However, there are many limitations on the use of free chitosanase in industrial production. Enzyme immobilization is generally considered a valuable strategy in industrial-scale applications. In this study, the chitosanase Csn-BAC from Bacillus sp. MD-5 was immobilized on Fe3O4-SiO2 magnetic nanoparticles (MNPs) to enhance its properties, which could be recovered easily from reaction media using magnetic separation. The activities of Csn-BAC immobilized with MNPs (MNPs@Csn-BAC) were determined with temperature and pH, and the thermal- and pH-stabilities, respectively. The reusability of the MNPs@Csn-BAC was determined in repeated reaction cycles. Immobilization enhanced the thermal and pH stability of Csn-BAC compared with the free enzyme. After eight reaction cycles using MNPs@Csn-BAC, the residual enzyme activity was 72.15%. Finally, the amount of COS released by MNPs@Csn-BAC was 1.86 times higher than that of the free Csn-BAC in the catalytic performance experiment. The immobilized Csn-BAC exhibits broad application prospects in the production of COS.

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