Functional characterization of rice OsDof12
文献类型: 外文期刊
第一作者: Li, Dejun
作者: Li, Dejun;Yang, Chunhua;Li, Xiaobing;Gan, Qiang;Zhao, Xianfeng;Zhu, Lihuang;Li, Dejun;Yang, Chunhua;Li, Xiaobing;Gan, Qiang;Zhao, Xianfeng;Zhu, Lihuang;Li, Dejun;Yang, Chunhua;Gan, Qiang
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关键词: Flowering time;OsDof12;Overexpression;Rice
期刊名称:PLANTA ( 影响因子:4.116; 五年影响因子:4.316 )
ISSN:
年卷期:
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收录情况: SCI
摘要: DNA-binding with one finger (Dot) proteins are a large family of transcription factors involved in a variety of biological processes in plants. In rice, 30 different Dof genes have been identified through genome analysis. Here we report the functional characteristics of a rice Dof gene, OsDof12, which encodes a predicted Dof protein. The nuclear localization of OsDof12 was investigated by the tran_sient expression assays of the OsDof12-GFP fusion protein in onion epidermal cells. Trans-activation assays in a yeast one-hybrid system indicated that OsDof12 had transcrip_tional activity. RNA expression analyses showed that the expression of OsDof12 was not tissue-specific in general and fluctuated at different development stages in rice. In addition, OsDof12 was strongly inhibited by dark treatments. The transgenic lines overexpressing OsDof12 showed early flow_ering under long-day (LD) conditions, whereas OsDof12 overexpression had no effect on flowering time under short-day (SD) conditions. In transgenic lines overexpressing OsDof12, the transcription levels of Hd3a and OsMADS14 were up-regulated under LD conditions but not SD conditions, whereas the expression of Hdl, OsMADS51, Ehdl and OsGI did not change under LD and SD conditions. These results suggested that OsDof12 might regulate flowering by control_ling the expression of Hd3a and OsMADS14.
分类号: 588E0003
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