Isolation via enrichment and characterization of 14 dinucleotide microsatellite loci in one catfish, Glyptosternum maculatum and cross-amplification in other related taxa

文献类型: 外文期刊

第一作者: Guo, Baoying

作者: Guo, Baoying;Xie, Congxin;Li, Hongjing;Shan, Lei;Wang, Huanling;Huo, Bin;Zhou, Qing;Zhang, Yan

作者机构:

关键词: Glyptosternum maculatum;Genetic diversity;Microsatellites;Cross-species amplification

期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )

ISSN:

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收录情况: SCI

摘要: A total of 14 novel dinucleotide microsatellite loci were developed using genomic DNA enrichment protocol and characterized for one Glyptosternoid catfish, Glyptosternum maculatum. Polymorphism was explored using 36 individuals from one natural population. The observed number of alleles ranged from 2 to 9. The ranges of observed and expected heterozygosity were 0.0278 to 1 and 0.4319 to 0.8498, respectively and the average of PIC was 0.5754. In addition, successful cross-species amplification of these set of microsatellite markers in the related taxa, Pseudechenneis sulcatus (McClelland) suggested that they would provide a useful tool for the genetic and conservation studies of Sisoridae species.

分类号: Q3

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