Molecular cytogenetic characterization of 9 populations of four species in the genus Polygonatum (Asparagaceae)

文献类型： 外文期刊

第一作者： Wei, Yan-Fang

作者： Wei, Yan-Fang;She, Chao-Wen;Jiang, Xiang-Hui;Song, Rong

作者机构：

关键词： Cytotaxonomy; fluorescence in situ hybridization; karyotype; karyotype asymmetry; Polygonatum; ribosomal RNA genes (rDNA)

期刊名称：COMPARATIVE CYTOGENETICS （ 影响因子：1.0； 五年影响因子：1.0 ）

ISSN： 1993-0771

年卷期： 2024 年 18 卷

页码：

收录情况： SCI

摘要： To characterize the chromosomes of the four species of Polygonatum Miller, 1754, used in traditional Chinese medicine, P. cyrtonema Hua, 1892, P. kingianum Collett et Hemsley, 1890, P. odoratum (Miller, 1768) Druce, 1906, and P. sibiricum Redout & eacute;, 1811, and have an insight into the karyotype variation of the genus Polygonatum , fluorescence in situ hybridization (FISH) with 5S and 45S rDNA oligonucleotide probes was applied to analyze the karyotypes of 9 populations of the four species. Detailed molecular cytogenetic karyotypes of the 9 populations were established for the first time using the dataset of chromosome measurements and FISH signals of 5S and 45S rDNA. Four karyotype asymmetry indices, CVCI , CVCL , M-CA and Stebbins' category, were measured to elucidate the asymmetry of the karyotypes and karyological relationships among species. Comparison of their karyotypes revealed distinct variations in the karyotypic parameters and rDNA patterns among and within species. The basic chromosome numbers detected were x = 9, 11 and 13 for P. cyrtonema , x = 15 for P. kingianum , x = 10 and 11 for P. odoratum , and x = 12 for P. sibiricum . The original basic chromosome numbers of the four species were inferred on the basis of the data of this study and previous reports. All the 9 karyotypes were of moderate asymmetry and composed of metacentric, submetacentric and subtelocentric chromosomes or consisted of two of these types of chromosomes. Seven populations have one locus of 5S rDNA and two loci of 45S rDNA, and two populations added one 5S or 45S locus. The karyological relationships among the four species revealed by comparison of rDNA patterns and PCoA based on x , 2n , TCL, CVCI , M(CA )and CVCL were basically accordant with the phylogenetic relationships revealed by molecular phylogenetic studies. The mechanisms of both intra- and inter -specific dysploidy in Polygonatum were discussed based on the data of this study and literature.

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