Data-Independent Acquisition Proteome Technology for Analysis of Antifungal and Anti-aflatoxigenic Properties of Eugenol to Aspergillus flavus

文献类型： 外文期刊

第一作者： Zhang, Xinyue

作者： Zhang, Xinyue;Zheng, Mumin;Yang, Bolei;Tai, Bowen;Li, Xu;Xing, Fuguo;Peng, Wenjing

作者机构：

关键词： Aspergillus flavus; aflatoxin B-1; eugenol; proteomics; secondarymetabolism

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 41 期

页码：

收录情况： SCI

摘要： Several toxicogenic Aspergilli, such as Aspergillus flavus and A. parasiticus, could biosynthesize aflatoxin B-1 (AFB(1)) and other mycotoxins. Chemical fungicides are commonly used to control fungal contamination, but chemical residues may pose significant risks to human health and environmental stability. Consequently, natural antifungal and aflatoxin-inhibiting agents could be sustainable alternatives. Eugenol has been used as an inhibitor of aflatoxins (AFs), which is a common essential oil. Nevertheless, the definite mechanism by which eugenol exerts its inhibitory effect on Aspergillus remains unclear. This research demonstrates that eugenol significantly suppressed fungi growth and AF production as the dose increased (40.9 to 100%). With the proteomics approach, the inhibition pathway of eugenol was investigated. The production of proteins involved in cell wall integrity was notably reduced under eugenol treatment, indicating that eugenol destroys the cell wall integrity of A. flavus. Furthermore, exposure to eugenol downregulated several fungal developmental regulators and subsequently inhibited A. flavus development. Energy metabolism in A. flavus is closely related to its secondary metabolism. Under eugenol treatment, the synthesis of proteins relevant to the pentose phosphate pathway was significantly enhanced, leading to a decrease in the availability of acetyl-CoA, a precursor for AF biosynthesis. Simultaneously, the valine, leucine, and isoleucine biosynthesis pathways were enhanced, further reducing the content of acetyl-CoA. This might be the primary factor in the inhibition of AF biosynthesis by eugenol. Ribosome biogenesis was the most dysregulated pathway based on KEGG data, indicating that eugenol disturbed ribosome biogenesis and affected its normal function in A. flavus. In conclusion, eugenol inhibits the cellular integrity, energy metabolism, and protein synthesis and then suppresses A. flavus development and AF biosynthesis, which provides a clearer grasp of the inhibitory mechanism meaningful for A. flavus and AF contamination control.

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