GhWRKY40 Interacts with an Asparaginase GhAPD6 Involved in Fiber Development in Upland Cotton (Gossypium hirsutum L.)

文献类型： 外文期刊

第一作者： Zhang, Sujun

作者： Zhang, Sujun;Cai, Xiao;Wang, Haitao;Liu, Cunjing;Li, Xinghe;Tang, Liyuan;Zhou, Xiaodong;Zhang, Jianhong;Zhang, Sujun;Cai, Xiao;Wang, Haitao;Liu, Cunjing;Li, Xinghe;Tang, Liyuan;Zhou, Xiaodong;Zhang, Jianhong;Wei, Jingyan

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关键词： fiber quality; GhWRKY40; aspartyl protease; yeast two-hybrid assay; split-luciferase complementation assay; Gossypium hirsutum

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2024 年 15 卷 8 期

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收录情况： SCI

摘要： Fiber quality improvement is a primary goal in cotton breeding. Identification of fiber quality-related genes and understanding the underlying molecular mechanisms are essential prerequisites. Previously, studies determined that silencing the gene GhWRKY40 resulted in longer cotton fibers; however, both the underlying mechanisms and whether this transcription factor is additionally involved in the regulation of cotton fiber strength/fineness are unknown. In the current study, we verified that GhWRKY40 influences the fiber strength, fiber fineness, and fiber surface structure by using virus-induced gene silencing (VIGS). Potential proteins that may interact with the nucleus-localized GhWRKY40 were screened in a yeast two-hybrid (Y2H) nuclear-system cDNA library constructed from fibers at 0, 10, and 25 days post-anthesis (DPA) in two near-isogenic lines differing in fiber length and strength. An aspartyl protease/asparaginase-related protein, GhAP(D6), was identified and confirmed by Y2H and split-luciferase complementation assays. The expression of GhAP(D6) was approximately 30-fold higher in the GhWRKY40-VIGS lines at 10 DPA and aspartyl protease activity was significantly upregulated in the GhWRKY40-VIGS lines at 10-20 DPA. This study suggested that GhWRKY40 may interact with GhAP(D6) to regulate fiber development in cotton. The results provide a theoretical reference for the selection and breeding of high-quality cotton fibers assisted by molecular technology.

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