Extraction and purification of grape seed polysaccharides using pH-switchable deep eutectic solvents-based three-phase partitioning

文献类型： 外文期刊

第一作者： Chen, Xueying

作者： Chen, Xueying;Tan, Zhijian;Wang, Runping

作者机构：

关键词： Deep eutectic solvents; Extraction and separation; Grape seed polysaccharides; Recyclable extractant; Three-phase partitioning

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 412 卷

页码：

收录情况： SCI

摘要： T-butanol is widely used in three-phase partitioning (TPP), which is harmful to the environment. pH-switchable deep eutectic solvents (DESs) can be used as recyclable alternatives to t-butanol. This study aimed to construct DES-based TPP for extracting and purifying grape seed polysaccharides (GSP). The main influence factors were investigated in single-factor experiments. DES-1 (dodecanoic acid: octanoic acid = 1:1)-based extraction was screened, and the extraction yield reached the maximum of 98.04 mg/g under the optimal conditions. Furthermore, DES can be recycled, only suffering a small loss capacity in extraction yield after 25 cycles. Most importantly, the extractability of DES could be completely recovered after switching and regeneration. The molecular weight of obtained GSP was 60 kDa, and the main monosaccharides of GSP included mannose, glucose, galactose, and arabinose. This study provides an efficient and sustainable method for the extraction of bioactive substances.

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