Identification and characterization of a novel Waxy allele from a Yunnan rice landrace

文献类型： 外文期刊

第一作者： Liu, Linglong

作者： Liu, Linglong;Ma, Xiaodong;Liu, Shijia;Zhu, Changlan;Jiang, Ling;Wang, Yihua;Shen, Yi;Ren, Yulong;Dong, Hui;Chen, Liangming;Liu, Xi;Zhao, Zhigang;Wan, Jianmin;Zhai, Huqu;Wan, Jianmin

作者机构：

关键词： Rice (Oryza sativa L.);Waxy gene;Granule-bound starch synthase (GBSSI);Amylose content;dCAPS marker

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Low amylose content (AC) is a desirable trait for rice (Oryza sativa L.) cooking quality and is selected in soft rice breeding. To gain a better understanding of the molecular mechanism controlling AC formation, we screened 83 Yunnan rice landraces in China and identified a rice variety, Haopi, with low AC. Genetic analyses and transgenic experiments revealed that low AC in Haopi was controlled by a novel allele of the Wx locus, Wxhp, encoding a granule-bound starch synthase (GBSSI). Sequence comparisons of Wxhp and Wxb alleles (from Nipponbare) showed several nucleotide changes in the upstream regulatory regions (including the promoter, 50-untranslated region, and first intron 50 splicing junction site). Interestingly, these changes had no obvious effect on the expression level and splicing efficiency of Wx transcripts. In addition, an examination of the coding region revealed that the Wxhp allele carries an A-to-G change at nucleotide position ?497 from the start codon, resulting in an Asp165/Gly165 substitution. The amino acid substitution had no detectable effects on GBSSI activity in vitro; however, it notably reduced the binding of GBSSI to starch granules, resulting in a reduction of AC in rice seeds. Moreover, three other Yunnan landraces with low AC also carry a nucleotide substitution identical to Haopi at the ?497 position of the Wx gene, suggesting common ancestry. Based on the single-nucleotide polymorphism, we have developed a new derived cleaved amplified polymorphic sequence marker for use in breeding practice to manipulate AC in rice endosperm.

分类号： Q946

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