Auxin regulates source-sink carbohydrate partitioning and reproductive organ development in rice

文献类型： 外文期刊

第一作者： Zhao, Zhigang

作者： Zhao, Zhigang;Wang, Chaolong;Yu, Xiaowen;Tian, Yunlu;Wang, Wenxin;Zhang, Yunhui;Bai, Wenting;Zheng, Hai;Wang, Qiming;Lu, Jiayu;Lei, Dekun;He, Xiaodong;Chen, Keyi;Gao, Junwen;Liu, Xi;Liu, Shijia;Jiang, Ling;Wan, Jianmin;Wang, Haiyang;Wan, Jianmin;Yang, Ning;Zhang, Tao;Yang, Ning;Zhang, Tao

作者机构：

关键词： auxin; source-sink; carbohydrate partitioning; reproductive organ; rice

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：12.779； 五年影响因子：13.45 ）

ISSN： 0027-8424

年卷期： 2022 年 119 卷 36 期

页码：

收录情况： SCI

摘要： Carbohydrate partitioning between the source and sink tissues plays an important role in regulating plant growth and development. However, the molecular mechanisms regulating this process remain poorly understood. In this study, we show that elevated auxin levels in the rice dao mutant cause increased accumulation of sucrose in the photosynthetic leaves but reduced sucrose content in the reproductive organs (particularly in the lodicules, anthers, and ovaries), leading to closed spikelets, indehiscent anthers, and parthenocarpic seeds. RNA sequencing analysis revealed that the expression of AUXIN RESPONSE FACTOR 18 (OsARF18) and OsARF2 is significantly up- and down-regulated, respectively, in the lodicule of dao mutant. Overexpression of OsARF18 or knocking out of OsARF2 phenocopies the dao mutant. We demonstrate that OsARF2 regulates the expression of OsSUT1 through direct binding to the sugar-responsive elements (SuREs) in the OsSUT1 promoter and that OsARF18 represses the expression of OsARF2 and OsSUT1 via direct binding to the auxin-responsive element (AuxRE) or SuRE in their promoters, respectively. Furthermore, overexpression of OsSUT1 in the dao and Osarf2 mutant backgrounds could largely rescue the spikelets' opening and seed-setting defects. Collectively, our results reveal an auxin signaling cascade regulating source-sink carbohydrate partitioning and reproductive organ development in rice.

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