Pathogenicity and immunogenicity of gI/gE/TK-gene-deleted Felid herpesvirus 1 variants in cats

文献类型： 外文期刊

第一作者： Tang, Aoxing

作者： Tang, Aoxing;Zhu, Meng;Zhu, Jie;Zhang, Da;Zhu, Shiqiang;Wang, Xiao;Meng, Chunchun;Li, Chuangfeng;Liu, Guangqing;Zhu, Meng

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关键词： Felid herpesvirus 1; CRISPR/Cas9; Recombinant virus; Vaccine; Cat

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.8； 五年影响因子：3.9 ）

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年卷期： 2023 年 20 卷 1 期

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收录情况： SCI

摘要： Background Felid herpesvirus 1 (FHV-1) is a major pathogenic agent of upper respiratory tract infections and eye damage in felines worldwide. Current FHV-1 vaccines offer limited protection of short duration, and therefore, do not reduce the development of clinical signs or the latency of FHV-1. Methods To address these shortcomings, we constructed FHV Delta gIgE-eGFP, FHV Delta TK mCherry, and FHV Delta gIgE/TK eGFP-mCherry deletion mutants (Delta gI/gE, Delta TK, and Delta gIgE/TK, respectively) using the clustered regularly interspaced palindromic repeats (CRISPR)/CRISP-associated protein 9 (Cas9) system (CRISPR/Cas9), which showed safety and immunogenicity in vitro. We evaluated the safety and efficacy of the deletion mutants administered with intranasal (IN) and IN + subcutaneous (SC) vaccination protocols. Cats in the vaccination group were vaccinated twice at a 4-week interval, and all cats were challenged with infection 3 weeks after the last vaccination. The cats were assessed for clinical signs, nasal shedding, and virus-neutralizing antibodies (VN), and with postmortem histological testing. Results Vaccination with the gI/gE-deleted and gI/gE/TK-deleted mutants was safe and resulted in significantly lower clinical disease scores, fewer pathological changes, and less nasal virus shedding after infection. All three mutants induced virus-neutralizing antibodies after immunization. Conclusions In conclusion, this study demonstrates the advantages of FHV-1 deletion mutants in preventing FHV-1 infection in cats.

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