Mass spectrometry-based proteomic landscape of rice reveals a post-transcriptional regulatory role of N6-methyladenosine

文献类型： 外文期刊

第一作者： Li, Shang-Tong

作者： Li, Shang-Tong;Ke, Yunzhuo;Zhu, Yunke;Huang, Huanwei;Li, Linxia;Hou, Zhiyang;Zhang, Xuemin;Li, Yaping;Liu, Chaofan;Li, Xiulan;Gu, Xiaofeng;Liang, Zhe;Li, Shang-Tong;Xie, Mengjia;Zhu, Tian-Yi;Zhou, Lianqi;Yang, Bing;Meng, Chen;Wang, Faming;Yu, Hao;Yu, Hao

作者机构：

期刊名称：NATURE PLANTS （ 影响因子：15.8； 五年影响因子：17.1 ）

ISSN： 2055-026X

年卷期： 2024 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Rice is one of the most important staple food and model species in plant biology, yet its quantitative proteomes are largely uncharacterized. Here we quantify the relative protein levels of over 15,000 genes across major rice tissues using a tandem mass tag strategy followed by intensive fractionation and mass spectrometry. We identify tissue-specific and tissue-enriched proteins that are linked to the functional specificity of individual tissues. Proteogenomic comparison of rice and Arabidopsis reveals conserved proteome expression, which differs from mammals in that there is a strong separation of species rather than tissues. Notably, profiling of N-6-methyladenosine (m(6)A) across the rice major tissues shows that m(6)A at untranslated regions is negatively correlated with protein abundance and contributes to the discordance between RNA and protein levels. We also demonstrate that our data are valuable for identifying novel genes required for regulating m(6)A methylation. Taken together, this study provides a paradigm for further research into rice proteogenome.

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