LuxS modulates motility and secretion of extracellular protease in fish pathogen Vibrio harveyi

文献类型： 外文期刊

第一作者： Zhang, Ya-Qiu

作者： Zhang, Ya-Qiu;Deng, Yi-Qin;Feng, Juan;Hu, Jian-Mei;Chen, Hao-Xiang;Guo, Zhi-Xun;Su, You-Lu;Zhang, Ya-Qiu;Hu, Jian-Mei;Chen, Hao-Xiang;Su, You-Lu

作者机构：

关键词： Vibrio harveyi; luxS; motility; flagella; extracellular protease secretion

期刊名称：CANADIAN JOURNAL OF MICROBIOLOGY （ 影响因子：3.226； 五年影响因子：2.731 ）

ISSN： 0008-4166

年卷期： 2022 年 68 卷 3 期

页码：

收录情况： SCI

摘要： Vibrio harveyi can cause infections and diseases in a variety of marine vertebrates and invertebrates, which are harmful to the aquaculture industry. The LuxS quorum-sensing system regulates the expression of virulence factors in a wide variety of pathogenic bacteria. In this study, an in-frame deletion of the luxS gene was constructed to reveal the role of LuxS in the physiology and virulence of V. harveyi. Statistical analysis showed no significant differences in the growth ability, biofilm formation, antibiotic susceptibility, virulence by intraperitoneal injection, and ability of V. harveyi to colonize the spleen and liver of the pearl gentian grouper between the wild-type (WI) and luxS mutant. However, deletion of luxS decreased the secretion of extracellular protease, while increasing swimming and swarming abilities. Simultaneously, a luxS-deleted mutant showed overproduction of lateral flagella, and an intact luxS complemented this defect. Since motility is flagella dependent, 16 V. harveyi flagella biogenesis related genes were selected for further analysis. Based on quantitative real-time reverse transcription-PCR (qRT-PCR), the expression levels of these genes, including the polar flagella genes flaB, flhA, flhF, flth, flhF, fliS, and flrA and the lateral flagella genes figA, flgB, fliE, fliF, lafA, lafK, and motY, were significantly upregulated in the Delta luxS: pMMB207 (Delta luxS(+)) strain as compared with the V. harveyi 345: pMMB207 (WT+) and C-Delta luxS strains during the early, mid-exponential, and stationary growth phases. Our results indicate that LuxS plays an important role in controlling motility, flagella biogenesis, and extracellular protease secretion in V. harveyi.

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