Structural elucidation, binding sites exploration and biological activities of bound phenolics from Radix Puerariae Thomsonii

文献类型： 外文期刊

第一作者： Li, Weixin

作者： Li, Weixin;Zhang, Min;Zhang, Ruifen;Huang, Fei;Dong, Lihong;Jia, Xuchao;Zhang, Mingwei

作者机构：

关键词： Radix puerariae thomsonii; Bound phenolics; Antioxidant activity; Alcohol -metabolizing enzymes; Carbohydrate -hydrolyzing enzymes; Stepwise enzymatic hydrolysis

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2024 年 450 卷

页码：

收录情况： SCI

摘要： Radix puerariae thomsonii (RPT) contains many phenolics and exhibits various health benefits. Although the free phenolics in RPT have been identified, the composition and content of bound phenolics, which account for approximately 20% of the total phenolic content, remain unknown. In this study, 12 compounds were isolated and identified from RPT-bound phenolic extracts, of which 2 were novel and 6 were reported first in RPT. ORAC and PSC antioxidant activities of 12 compounds, as well as their effects on alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), alpha-glucosidase, and alpha-amylase were evaluated. Genistein exhibited the highest ORAC activity, while daidzin demonstrated superior PSC activity. Five compounds, including two new compounds, exhibited the ability to activate both ADH and ALDH. All the compounds except 4-hydroxyphenylacetic acid methyl ester and 2,4,4 '-trihydroxydeoxybenzoin demonstrated inhibitory effects on alpha-glucosidase and alpha-amylase. Alkaline hydrolysis and stepwise enzymatic hydrolysis revealed that bound phenolics in RPT mainly exist within starch.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序