A novel glycosyltransferase from Bacillus subtilis achieves zearalenone detoxification by diglycosylation modification

文献类型： 外文期刊

第一作者： Zhou, Yuqun

作者： Zhou, Yuqun;Yang, Jiguo;Tang, Yuqian;Yang, Jiguo;Yu, Yuanshan

作者机构：

期刊名称：FOOD & FUNCTION （ 影响因子：6.1； 五年影响因子：6.5 ）

ISSN： 2042-6496

年卷期： 2024 年 15 卷 11 期

页码：

收录情况： SCI

摘要： Zearalenone (ZEN), a nonsteroidal estrogenic mycotoxin produced by Fusarium spp., contaminates cereals and threatens human and animal health by inducing hepatotoxicity, immunotoxicity, and genotoxicity. In this study, a new Bacillus subtilis strain, YQ-1, with a strong ability to detoxify ZEN, was isolated from soil samples and characterized. YQ-1 was confirmed to degrade more than 46.26% of 20 mu g mL(-1) ZEN in Luria-Bertani broth and 98.36% in fermentation broth within 16 h at 37 degrees C; one of the two resulting products was ZEN-diglucoside. Under optimal reaction conditions (50 degrees C and pH 5.0-9.0), the reaction mixture generated by YQ-1 catalyzing ZEN significantly reduced the promoting effect of ZEN on MCF-7 cell proliferation, effectively eliminating the estrogenic toxicity of ZEN. In addition, a new glycosyltransferase gene (yqgt) from B. subtilis YQ-1 was cloned with 98% similarity to Bs-YjiC from B. subtilis 168 and over-expressed in E. coli BL21 (DE3). ZEN glycosylation activity converted 25.63% of ZEN (20 mu g mL(-1)) to ZEN-diG after 48 h of reaction at 37 degrees C. The characterization of ZEN degradation by B. subtilis YQ-1 and the expression of YQGT provide a theoretical basis for analyzing the mechanism by which Bacillus spp. degrades ZEN.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序