Bacillus amyloliquefaciens LM-1 Affects Multiple Cell Biological Processes in Magnaporthe oryzae to Suppress Rice Blast

文献类型： 外文期刊

第一作者： Liang, Meiling

作者： Liang, Meiling;Feng, Aiqing;Wang, Congying;Zhu, Xiaoyuan;Su, Jing;Yang, Jianyuan;Wang, Wenjuan;Chen, Kailing;Chen, Bing;Lin, Xiaopeng;Feng, Jinqi;Chen, Shen;Xu, Zihan

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关键词： Magnaporthe oryzae; Bacillus amyloliquefaciens LM-1; appressorium; cell death; autophagy

期刊名称：MICROORGANISMS （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 12 卷 6 期

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收录情况： SCI

摘要： Magnaporthe oryzae, one of the most destructive rice pathogens, causes significant losses during the rice harvest every year. Bacillus amyloliquefaciens has been explored in many crops as a potential biocontrol agent. However, the mechanisms of B. amyloliquefaciens controled rice blast are not fully understood. Here, a biocontrol strain LM-1, isolated from a contaminated medium, was identified as B. amyloliquefaciens using morphological observation, physiological and biochemical tests, and 16S rDNA sequencing. LM-1 inhibited the growth and pathogenicity of M. oryzae and Bipolaris oryzae (Breda de Haan) Shoem. The mycelia of M. oryzae co-cultured with LM-1 were enlarged and broken by fluorescence microscopy using calcofluor white. LM-1 inhibited the mycelia of M. oryzae from producing conidia. Genes itu, srf, and fenB were detected in LM-1. Furthermore, the supernatant of LM-1 interfered with the appressorium formation of M. oryzae, blocked conidial cell death, and reduced autophagy degradation but did not affect the normal germination of rice seeds and seeding growth. Additionally, we observed hypersensitivity reactions, reactive oxygen species, and iron accumulation reduction in rice cells inoculated with supernatant. Our study reveals that LM-1 has a control effect on rice blast and affects cell wall integrity, sporulation, appressorium formation, cell death, and autophagy.

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