Establishment of a novel engineering breeding technology for cytoplasmic male sterility

文献类型： 外文期刊

第一作者： Xu, Na

作者： Xu, Na;Yuan, Zheming;Yu, Dong;Zhang, Longhui;Wang, Tiankang;Hu, Zhongxiao;Song, Shufeng;Li, Li;Xu, Na;Yu, Dong;Song, Shufeng;Li, Li;Lei, Quanqiang;Qiu, Ngxin;Song, Shufeng;Li, Li

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关键词： Three-line hybrid rice; CMS-WA; Rf gene; CEBT; Reproductive lines

期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）

ISSN： 2095-5421

年卷期： 2025 年 13 卷 4 期

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收录情况： SCI

摘要： The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20, sorting marker gene DsRed2, and pollen lethal gene ZmAA1, then transformed this into a CMS line. We obtained reproductive lines without alterations to main agronomic traits, which can pro-duce engineering sterile lines (ESL) and reproductive line seeds at a 1:1 ratio by self-crossing. CEBT con-verts the traditional three-line system into an efficient 'new two-line' hybrid framework: the reproductive line is improved while simultaneously improving the ESL, which significantly shortens the breeding process for three-line hybrid rice. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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